怀牛膝总皂苷对大鼠血管平滑肌细胞内bFGF和ICAM-1表达的影响

Effect of Achyranthes Bidentata Saponins on bFGF and ICAM-1 of Rats' Aorta Vascular Smooth Muscle Cells

目的:探讨怀牛膝总皂苷(ABS)对大鼠血管平滑肌细胞(VSMC)内细胞因子的影响,阐明其抗动脉粥样硬化的机制。方法:采用氧化型低密度脂蛋白(ox-LDL)诱导平滑肌细胞增殖的方法,以GAPDH作内标,RT-PCR检测相关细胞因子的表达量,评价ABS对细胞内碱性成纤维细胞生长因子(bFGF)和细胞间黏附分子(ICAM-1)mRNA表达量的影响。结果:ox-LDL浓度为30 mg/L促进VSMC增殖最强;ABS(50、100、150mg/L)显著抑制ox-LDL诱导VSMC内bFGF、ICAM-1基因表达,作用呈浓度依赖性。结论:ABS能抑制在ox-LDL作用下VSMC内细胞因子的表达,说明其具有抗动脉粥样硬化的作用。

Objective ： To investigate the influence of Achyranthes bidentata saponins （ABS） on the cytokines of rats＇ a- orta vascular smooth muscle cells, and to elucidate its mechanism of anti - atherosclerosis. Methods ： The oxidized low den- sity lipoprotein （ ox - LDL） was used to induce the proliferation of rats＇aorta vascular smooth muscle cells （VSMC）. The effects of different concentrations of ABS on the cytokines of VSMC induced by ox - LDL were observed. RT - PCR meth- od was used to detect the expressions of bFGF and ICAM mRNA with GAPDH as internal standard. Result ： The prolifera- tion of VSMC was at the highest level when the concentration of ox - LDL was 30 mg/L, and this effect of ox - LDL could be significantly inhibited by ABS （50,100,150 mg/L） in a concentration -dependent manner. ABS could also inhibit the expressions of bFGF and ICAM - 1 mRNA of VSMC. Conclusion ： ABS could inhibit the expressions of cytokines of VSMC induced by ox - LDL. From these results,it shows that ABS has anti - atherosclerosis effect.