大肠癌组织中核转录因子-κB p65及磷酸酶和张力蛋白同源缺失基因的表达及意义

The expression and significance of nuclear transcription factor-κB p65 and PTEN in colorectal cancer

目的探讨大肠癌中核转录因子NF-κB p65和抑癌基因磷酸酶和张力蛋白同源缺失基因(PTEN)的表达及意义。方法采用免疫组织化学方法检测59例大肠癌组织中,NF-κB和抑癌基因PTEN的表达。结果 59例大肠癌组织中NF-κBp65和PTEN阳性表达率分别为44.1%(26/59)和37.3%(22/59)。两者表达呈负相关(P<0.05)。大肠癌组织中PTEN的表达与肠周淋巴结转移显著相关(P<0.05),NF-κBp65核表达与肠周淋巴结转移无关(P>0.05)。不同病理类型、肿瘤侵袭程度、性别、年龄的大肠癌组织中,NF-κBp65核表达与PTEN的表达差异无统计学意义(P>0.05)。结论 NF-κBp65的激活与PTEN基因缺失均参与了大肠癌的发生发展及演变。

Objective To study the expression and significance of NF-κBp65 and tumor suppressor gene PTEN in colorectal cancer. Methods Immunohistochemical staining was used to detect the positive expression of NF-κBp65 and PTEN gene in 59 colorectal cancer specimens. Resluts In 59 coloreetal cancer specimens screened, the positive expression rate of NF-κBp65 and PTEN were 44. 1% （ 26/59 ） and 37.3% （22/59）. In 59 eoloreetal cancer specimens screened,the negative expression rate of NF-κBp65 and PTEN were 55.9% （33/59）and 62. 7% （37/59）. There was a negative correlation between positive expression of NF-κBp65 and PTEN （P 〈 0. 05 ）. There was significant negative correlation between PTEN gene positive expression and lymph node metastases （ P 〈 0. 05 ）. There was no significant relationship between NF-κBp65 positive expression and lymph node metastases （ P 〉 0. 05 ）. NF-κBp65 nuclear positive expression and PTEN gene positive expression were no significant difference in different pathological types, the depth of invasion, sex and age. （ P 〉 0. 05 ）. Conclusions The activation of NF--κBp65 and PTEN loss are associ- ated with the pathogenesis and development of eolorectal cancer.