STARD3基因单核苷酸多态性及蛋白质表达与胃癌风险性

Association analysis of STARD3 genetic polymorphisms and expression with gastric cancer risk

目的观察STARD3基因单核苷酸多态性与汉族人群胃癌易感性关系，探讨STARD3表达与胃癌临床病理特征相关性。方法采用Sequenom基因分型仪对安徽地区311例胃癌患者及425例正常对照组血样STARD3rs9972882、rs881844、rs11869286和rs1877031多态性进行基因分型。采用组织芯片及免疫组化法对其中243例胃癌组织和20例癌旁正常胃黏膜的STARD3表达进行检测。结果STARD3基因4个单核苷酸多态性在胃癌和对照组之间分布差异无显著性。单倍型分析提示STARD3单倍型CCCT连锁影响胃癌易感性（P=0．043，OR=0．805，95％CI=0．643—0．992）。临床病理资料分层分析显示STARD3rs1877031杂合子Tc更多见于胃黏液腺癌和印戒细胞癌（P=0．021，OR=2．882，95％CI=1．173—7．084）。免疫组化结果发现胃癌组织STARD3表达与患者性别、饮酒、肿瘤部位、组织学类型和分化程度密切相关（P〈0．05）。结论STARD3可能与中国人群胃癌的发生、分化程度及组织学形态相关。

Purpose To investigate the association of single nucleotide polymorphisms （SNPs） of STARD3 and its expression with risk of gastric cancer （GC） in the Chinese Han population. Methods STARD3 SNPs： rs9972882, rs881844, rs11869286 and rs1877031 in 311 gastric cancers and 425 cancer-free controls in Anhui area were performed by using Sequenom. Expression of STARD3 in 243 cases of the gastric cancer and 20 cases of adjacent noncancerous normal gastric mucosa were detected by immumohistochemieal （IHC） analysis of tissue microarrays （TMA）. Results There were no associations between genetic variations of STAR3 gene and GC risk in the Chinese Han population. However, the haplotype CCCT of STARD3 variants might have a synergetic effect on the susceptibility to GC （P = 0. 043, OR = 0. 805, 95% CI = 0. 643 -0. 992）. The STARD3 rs1877031 TC genotype was predominantly observed in gastric mucinous adenocarcinoma and signet-ring cell carcinoma （P = 0. 021, OR = 2. 882, 95% CI = 1. 173 - 7.084）. Expression of STARD3 significantly correlated with gender, alcohol drinking, location of primary tumor, histological type and differentiation （ P 〈 0.05 ）. Conclusion STARD3 would be related to the tumorigenesis, differentiation degree and histological type of GC in the Chinese popula- tion.