维生素C、E对慢性酒精中毒小鼠学习记忆的影响

Effects of Vitamin C and Vitamin E on learning and memory in mice with chronic alcoholism

目的观察维生素C(VC)、维生素E(VE)及其合用对慢性酒精中毒小鼠学习记忆能力的影响。方法昆明种小鼠65只,雌雄不拘,随机分成5组,每组13只,各组分别给予生理盐水、乙醇、乙醇+VC、乙醇+VE、乙醇+VC+VE灌胃21天后,各组均取10只,用Morris水迷宫检测其学习记忆能力,记录潜伏期与穿越次数。结果乙醇组与生理盐水组比较潜伏期延长、穿越次数减少,学习记忆能力降低;乙醇+VC组、乙醇+VE组及乙醇+VC+VE组与乙醇组比较潜伏期均缩短,乙醇+VC+VE组穿越次数增加,表明VC、VE对乙醇中毒小鼠学习记忆能力有改善作用,且VC、VE合用有协同作用。结论慢性酒精中毒可致学习记忆能力下降;VC、VE可改善酒精中毒小鼠的学习记忆能力,二者合用具有协同保护作用。

Objective To respectively observe the effects of Vitamin C （VC）, Vitamin E （VE） and their joint use on learning and memory in mice with chronic alcoholism. Methods 65 Kunming mice of both sexes were randomly divided into 5 groups. Each group were implemented gastric irrigation of normal saline （NS） , alcohol, alcohol ＋ VC, alcohol ＋ VE, alcohol ＋ VC ＋ VE respectively for 21 days. Learning and memory of 10 mice from each group were evaluated with a series of tasks to find a hidden platform in Morris water maze, escape latencies and crossing times recorded. Results The escape latencies of mice with alcohol treatment were statistically longer, crossing times fewer, compared with those with NS treatment, suggesting the negative effects of alcohol on learning and memory of mice; the escape latencies of mice with alcohol ＋ VC treatment and with alcohol ＋ VE treatment and with alcohol ＋ VC ＋ VE treatment were all statistically shorter, crossing times of mice with alcohol ＋ VC ＋ VE treatment more, compared with those of alcohol treatment, demonstrating the important role of VC and VE in enhancing learning and memory of mice with alcoholism; the escape latencies of mice with alcohol ＋ VC ＋ VE treatment were statistically shorter, compared with those with alcohol ＋ VC treatment and with alcohol ＋ VE treatment, showing the synergistic effects of the joint use of VC and VE in this case. Conclusions Chronic alcoholism could undermine the learning and memory; the use of VC or VE could improve the learning and memory ability of mice with chronic alcoholism, the joint use of VC and VE achieve svnergic effects.