摘要
目的 探讨高危型人乳头状瘤病毒（HPV）感染和肿瘤转移抑制蛋白KAIl在子宫颈癌形成及进展中的意义.方法 对117例石蜡包埋子宫颈组织[正常对照20例、子宫颈上皮内瘤样病变（CIN）58例、子宫颈癌39例]采用免疫组织化学SP法检测KAI1蛋白、HPV16/18E6、HPV16E7蛋白的表达情况.结果 在正常子宫颈组织、CIN和子宫颈癌组织中,KAII阳性表达率分别为90.0％（18/20）、72.4％（42/58）、25.6％（10/39）,呈下降趋势,差异有统计学意义（P＜0.01）. HPVI6/18E6阳性表达率分别为0（0/20）、31.0％（18/58）、41.0％（16/39）,差异有统计学意义（P＜ 0.01）；HPV16E7阳性表达率分别为0（0/20）、34.5％（20/58）、64.1％（25/39）,差异有统计学意义（P＜0.01）.但KAI1与HPV16/18E6、HPV16E7阳性表达间无相关性（P=0.429）.KAI1蛋白在子宫颈癌中的阳性表达率与细胞分化程度、临床分期、淋巴结转移有关（均P＜ 0.05）,与发病年龄无关（P＞0.05）；HPV感染与子宫颈癌发病年龄、临床分期、细胞分化程度、淋巴结转移均无关（均P＞ 0.05）.结论 KAI1蛋白在子宫颈癌中的表达下调,HPV16/18E6、HPV16E7感染与KAI1蛋白表达无关.
Objective To evaluate the infection of high-risk human papillomavirus （HPV） and the expression of tumor metastasis suppressor protein KAI1 in cervical cancer.Methods The expression of KAH,HPV16/18E6 and HPV16E7 proteins were analyzed by immunohistochemistry SP assay in 117 cases of paraffin-embedded cervical tissue,including 20 normal cerival tissue as control,58 intraepithelial neoplasia （CIN） and 39 cervical cancer.Results In normal cervical tissue,CIN and cervical cancer,the positive rate of KAI1 protein were respectively 90.0 % （18/20）,72.4 % （42/58）,25.6 % （10/39）.There was significant differience among three gruop （P 〈 0.01）.The positive rate of HPV16/18E6 and HPV16E7 proteins were respectively 0 （0/20）,31.0 % （18/58）,41.0 % （16/39）; 0 （0/20）,34.5 % （20/58）,64.1% （25/39）.There were significant differience among three gruops （P 〈 0.01）.There was no correlation between the expression of KAI1 and the infection of HPV （P =0.429）.Expression of KAI1 was correlated to grade of differentiation,clinical stage and lymph node metastasis （P 〈 0.05）,but was not correlated to age （P 〉 0.05）.HPV infection was not correlated to the age,clinical stage,cell differentiation and lymph node metastasis （P 〉 0.05）.Conclusion The expression of KAI1 protein is down-regulated in cervical cancer,which is not associated with the infection of HPV.
出处
《肿瘤研究与临床》
CAS
2013年第10期693-695,699,共4页
Cancer Research and Clinic
