华蟾素注射液对与人肝癌HepG-2细胞共培养下人淋巴管内皮细胞的增殖及管状结构形成的影响

Effect of Cinobufacini Injection on Proliferation and Tube-Like Structure Formation of Human Lymphatic Endothelial Cells Co-Cultured with Human Hepatoma HepG-2 Cells

目的探讨华蟾素注射液对与人肝癌HepG-2细胞共培养下人淋巴内皮细胞增殖、迁移及管状结构形成的影响。方法采用Transwell方法建立HepG-2细胞与人淋巴内皮细胞共培养系统;通过绘制细胞生长曲线,观察华蟾素注射液对共培养系统中人淋巴内皮细胞增殖的影响;通过细胞迁移实验,观察华蟾素注射液对共培养系统中人淋巴内皮细胞迁移的影响;通过基质胶实验,观察华蟾素注射液对共培养系统中人淋巴内皮细胞管状结构形成的影响。结果华蟾素注射液能够显著抑制与HepG-2细胞共培养下人淋巴内皮细胞增殖(P<0.05),迁移(P<0.05)及管状结构形成(P<0.05),呈剂量依赖性。结论华蟾素注射液抑制与HepG-2细胞共培养下人淋巴内皮细胞增殖,迁移及管状结构形成,可能是华蟾素注射液抑制肿瘤淋巴道转移的机制之一。

OBJECTIVE To study the effect of cinobufacini injection on proliferation, migration and tube-like structure formation of human hepatoma HepG-2 cells co-cultured with human lymphatic endothelial cells. METHODS Co-culture system of human hepa- toma HepG-2 cells and human lymphatic endothelial cells is established by means of transwell chamber; cell growth curve is used to ob- serve the effect of cinobufacini injection on the proliferation of HLEC co-cuhured with HepG-2 cells ; migration assay is used to observe the effect of cinobufacini injection on the migration of HLEC co-cultured with HepG-2 ; matrigel assay is used to observe the effect of cinobufacini injection on the tube-like structure formation of HLEC co-cultured with HepG-2 cells. RESULTS Cinobufacini injection significantly inhibits proliferation（ P 〈 0. 05 ）, migration （ P 〈 0. 05 ） and tube-like structure formation （ P 〈 0. 05 ） of HLEC co-cultured with HepG-2 cells in dose dependent ways. CONCLUSION Cinobufacini injection inhibits proliferation, migration and tube-like structure formation of HLEC co-cultured with HepG-2 cells ,which might contribute the inhibiting mechanisms of cinobufacini injection on tumor metastasis.