荚果蕨贯众提取物中黄酮类成分的含量测定和抗氧化活性研究

Determination of the Contents of the Main Flavones and Antioxidative Activity of the Extract of Rhizomes and Frond Bases of Matteuccia struthiopteris

目的建立荚果蕨贯众提取物中3种主要黄酮类成分的含量测定方法,并对荚果蕨贯众提取物的抗氧化能力进行评价。方法采用HPLC测定提取物中黄酮类成分含量。色谱柱为Kromasil C18柱(4.6 mm×250 mm,5μm);流动相为甲醇-水(水中含0.1%磷酸)70∶30;流速为1.00 mL·min-1;检测波长295 nm;柱温40℃。采用DPPH自由基清除法评价提取物抗氧化活性。结果对照品去甲氧基荚果蕨素、荚果蕨素、荚果蕨黄素记为D1、D2、D3分别在0.058～0.58、0.045～0.45、0.003～0.028 mg·mL-1内线性关系良好,r分别为0.999 8、0.999 8、0.998 3,平均回收率分别为96.93%、97.15%、96.41%。以IC50值(清除率为50%时,抗氧化剂的浓度值)作为评价指标,测得荚果蕨贯众提取物的IC50值为0.55 mg·mL-1。结论此含量测定方法具有良好的准确性,重复性和稳定性,可用于荚果蕨贯众提取物的质量控制。该提取物具有一定的抗氧化活性。

OBJECTIVE To establish a method to determine three main flavones in the extract of rhizomes and frond bases of Matteuccia struthiopteris and evaluate the antioxidative activity of the extract. METHODS The contents of the flavones in the extract were determined by HPLC. Kromasil Ciscolumn was used. Methanol-0. 1% orthophosphoric acid solution （70： 30） was used as the mo- bile phase at a flow rate of 1.00 mL ·min - i. The detection wavelength was set at 295 nm and the column temperature was 40℃. The antioxidative activity of the extract was detected by DPPH free radical scavenging assay. IC50 （ the antioxidant concentration for scaven- ging half of DPPH radical） was used as the index to evaluate scavenging capacity. RESULTS The calibration curves of deme- thoxymatteucinol, matteucinol, and matteuorien showed good linearity over the ranges of 0. 058 - 0. 58, 0. 045 - 0. 45, and 0. 003 - 0. 028 mg ·mL-1 （r =0. 999 8, 0. 999 8, 0. 998 3） , respectively. The average recoveries were 96. 93% , 97. 15% , and 96.41%, respectively. The IC50 value of the extract of rhizomes and frond bases of Matteuccia struthiopteris was 0. 55 mg ·mL- 1. CONCLUSION The quantitative determination method with good accuracy, repeatability and stability is suitable for the quality control of the extract of rhizomes and frond bases of Matteuccia struthiopteris. The extract has potential antioxidative action.