解淀粉芽孢杆菌HF-01发酵条件优化

Optimizing Fermentation Condition for Bacillus amyloliquefaciens HF-01

为探讨解淀粉芽孢杆菌BacillusamyloliqruefaciensHF-01菌株液体发酵条件，提高其活性次级代谢产物的产量，以茵体生物量和发酵液拮抗柑桔绿霉病菌PenicilliumdigitatumSacc．活性为指标，采用单因子试验和正交设计方法对菌株的最适发酵培养基成分及发酵条件进行优化。结果表明，菌株HF-01最适发酵培养基为葡萄糖3．0％、酵母提取粉0．5％、氯化钠0_3％、硫酸镁0．1％；最佳发酵培养条件为初始pH值6．0～6．5、培养温度28℃、培养时间48h、250mL三角瓶装液量90mL、接种量体积分数6％、摇床转速130r·min-1。在最佳发酵培养基和培养条件下，菌株HF-01抑菌能力提高37．3％；25℃条件下，优化后所得发酵滤液处理柑橘果实4d后，发病率为31．7％，病斑直径为25．9mm，显著低于优化前所得发酵滤液的发病率56．7％和病斑直径48．1mm。

In order to investigate best condition for liquid fermentation of Bacillus amyloliquefaciens HF-01 and increase the antagonistic substance productivity of strain HF-01, the single factor experiments and orthogonal experiments were adopted to optimize the liquid fermentation medium and fermentation conditions. The optimum medium was 3.0% glucose, 0.5% yeast extract powder, 0.3% NaC1 and 0.1% MgSO4. The optimal fermentation conditions were initial pH 6.0--6.5, temperature 28℃, fermentation time 48 h, medium capacity 90 mL/250 mL, inoculation rate 6%, and rotate speed 130 r＇min-1. Inhibitory activity of strain HF-01 fermentation solution increased 37.3% under the optimum fermentation medium and culture conditions. When optimized filtrate was treated on citrus fruits under 25 ℃ for 4 days, the disease incidence and lesion diameter were 31.7% and 25.9 mm respectively, significantly lower than unoptimized filtrate with 56.7% disease incidence and 48.1 mm lesion diameter.