氯化锂对肝癌细胞BEL-7402增殖、周期的影响及其作用机制

Effect of lithium chloride on proliferation and cell cycle of human hepatoma cells

目的探讨氯化锂对人肝癌细胞BEL-7402增殖、周期的影响及其作用机制。方法应用MTT法及DNA PREP细胞周期法检测BEL-7402经氯化锂作用后的增殖及周期改变情况,Western blot检测糖原合成激酶-3(GSK-3)、失活pGSK-3及其下游分子CyclinD1的表达情况。结果氯化锂可显著抑制BEL-7402的增殖,并呈时间、剂量依赖性(P<0.05);氯化锂可使BEL-7402的G_0/G_1期比例明显降低、G_2/M期比例显著升高(P<0.05);氯化锂作用后GSK-3、失活pGSK-3及CyclinD1蛋白的表达水平均明显升高(P<0.05)。结论氯化锂可抑制肝癌细胞增殖,其机制可能是通过抑制GSK-3活性并上调CyclinD1表达使肝癌细胞阻滞于G_2/M期。

Objective To investigate the effect of lithium chloride （LiCI） on cell proliferation,cell cycle of human hepatoma cells and its mechanism. Methods Cultured human hepatoma BEL-7402 cells were treated with different concentrations of LiCI. Cell proliferation was measured by MTT assay and cell cycle was examined by DNA PREP cell cycle assay. The expressions of GSK-3, inactivated GSK-3 and CyclinD1 were determined by Western blot. Results LiCI inhibited BEL-7402 cell proliferation in a concentration- and time-dependent manner. After LiCI treatment the percentage of G0/G 1 phase was significantly reduced, the G2/M phase was significantly increased, and the expression levels of GSK-3, inactivated GSK-3 and CyclinD1 were up-regulat- ed. Conclusion LiCI can inhibit the proliferation of BEL-7402 cells, in which inactivation of GSK-3 and up-regulation of CyclinD1 may be involved leading to cell cycle arrest in G2/M phase.