摘要
结合生命科学中生物活性物质的高灵敏度分析检测方法研究 ,组装了时间分辨激光荧光免疫分析装置 ;合成了稀土离子与蛋白质联接的双向螯合剂 二乙三胺五乙环酐(DTPAA) ;纯化了抗 乙型肝炎表面抗体 ;研究了Eu3+ DTPAA 抗 乙型肝炎表面抗体制备过程 ;确定了标记物制备的最佳条件 ;依据解离增强原理 ,采用时间分辨激光荧光光谱技术 ,对Eu( β NTA) 3(TOPO) 2 螯合物的有关参数进行了测定。
A microsecond time resolved laser fluorescence spectroscopic analysis set was developed. A chelate cyclic anhydride of diethylenetrimin pentaacetic acid anhydride (DTPAA) was synthesized. An anti HBs antibody was purified. A Eu 3+ DTPAA anti HBs label was prepared by two step procedure. We described the optimal condtion with Eu 3+ as marker and DTPAA as chelate bounding to antibody molecule. Labeling parameters such as solvent pH, protein and chelate molar ratio, reaction time, separation method were discussed in detail.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2000年第11期1439-1442,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助课题(No.39870705)
