核磁共振研究蛋白二级结构的方法

Determination of the Secondary Structure of Proteins by Neuclear Mangnetic Resonance

核磁共振法常规应用于解析化学物质结构和反应性能 ,现已发展成为生物化学和分子生物学的重要实验技术 ,尤其是在确定溶液中蛋白和多肽结构方面起着其它物理分析方法不可比拟的作用。文章综述了化学位移 ,偶合常数 ,核 Overhauser效应 ( NOE)以及同位素交换等确定蛋白或多肽二级结构的方法。

Nuclear magnetic resonance is used to be applied to determine the structure and reaction activity of chemical substances. Now it has become an important experimental technique of biochemistry and molecular biology, especially for analyzing structure of protein and multipeptide in solution. This paper summarizes several methods to assign the secondary structure of protein and multipepitde, such as chemical shift, coupling constant and nuclear Overhauser effect.