S-腺苷蛋氨酸对大鼠肝脏部分缺血再灌注损伤的保护作用及机制研究

The protective effect and mechanism of S-ademetionine on hepatic partial ischemia-reperfusion injury in rats

目的:探讨S-腺苷蛋氨酸对大鼠肝脏部分缺血再灌注损伤的保护作用及机制。方法:将36只雌性SD大鼠随机分为3组(n=12):假手术组、缺血再灌注组、S-腺苷蛋氨酸组。建立大鼠肝脏70%缺血再灌注模型,缺血时间为1 h。腺苷蛋氨酸于术前1 d及术后1 h腹腔注射(100 mg/kg),假手术组及缺血再灌注组在相同时间注射等量生理盐水。各组大鼠分别于肝脏再灌注后6 h和24 h处死,取肝脏及血液标本,检测血清谷丙转氨酶(alanine aminotransferase,ALT)、谷草转氨酶(aspertate aminotransferase,AST)、肿瘤坏死因子-α(tumor necrosis factorα,TNF-α),以及肝脏组织丙二醛(malonyldialdehyde,MDA)含量,并观察肝脏组织的病理改变。结果:与缺血再灌注组相比,S-腺苷蛋氨酸组的ALT、AST、TNF-α水平明显降低(P<0.01),MDA含量明显减少(P<0.05),肝脏病理损伤较轻。结论:S-腺苷蛋氨酸对大鼠肝脏缺血再灌注损伤具有保护作用,其机制可能与抗氧化及抑制炎症因子产生和活化有关。

Objective： To investigate the protective effect and mechanism of S-ademetionine against hepatic ischemia-reperfusion injury in rats and its mechanism. Methods： Thirty-six SD female rats were randomly divided into 3 groups, SHAM： sham operation group, IR： ischemia-reperfusion group, SAMe： S-ademetionine treatment group. The model of 70% hepatic ischemia-reperfusion was established in the rats. Ischemia lasted for 1 h. The rats of SAMe group received SAM（100 mg/kg） intraperitoneally 1 d prior to operation and 1 h after operation. The other groups received saline solution at the same time. Rats were killed at 6,24 h after reperfusion respectively. Blood samples were collected and analyzed for the levels of serum, alanine aminotransferase（ALT）, aspertate aminotransferase（AST）, tumor necrosis factor α（TNF-α）. The malonyldialdehyde （MDA） in liver tissues were measured and histologic changes were observed in HE staining. Results： Comparison to IR group, SAMe group was associated with lower serum transaminases ALT, AST, TNF-α. Besides, MDA expression in SAMe group was significantly lower than those in IR group. The injury of liver tissue in IR group was more obviously. Conclusion： S- ademetionine has a protective effect in hepatic ischemia-reperfusion injury in rat. The mechanism of protection appears to be dependent on its inhibitory effect on neutrophil infiltration and inflammatory cytokines.