反相高效液相色谱法测定米根霉乳酸发酵液中的乳酸

Determination of Lactic Acid in Fermentation Broth of Rhizopus Oryzae by Reversed-Phase High Performance Liquid Chromatography (RP-HPLC)

采用反相高效液相色谱法测定了米根霉乳酸发酵液中的乳酸。在Wakosil Ⅱ 5C18RS (4 6mmi d ×15 0mm ,5 μm)色谱柱上 ,以 0 0 1mol/L磷酸溶液 (pH 2 5 )作流动相 ,流速为 1mL/min ,紫外检测波长为 2 10nm。实验结果表明 ,该方法的相对标准偏差为 0 2 2 % (n =5 ) ,回收率为 99%以上。方法简便、快速。

A method for determining lactic acid in fermentation broth of Rhizopus oryzae by RP HPLC is described The operating conditions were Wakosil Ⅱ 5 C\-\{18\} RS column(4 6 mm i d ×150 mm,5 μm)at room temperature, 0 01 mol/L phosphoric acid solution (pH 2 5) as mobile phase with a flow rate of 1 0 mL/min and UV detection at 210 nm. The retention time of lactic acid was 3 820 min This method is simple, rapid and accurate. The results will not be affected by other components in the broth The relative standard deviation was 0 22%( n =5), and the recovery was over 99%.