开管毛细管亲和液相色谱初探

The Preliminary Study on the Open Tubular Capillary Affinity Liquid Chromatography

提出了一种新的亲和色谱模式开管毛细管亲和液相色谱。在内径为 5 0 μm的毛细管内表面键合一层三嗪染料配体 ,利用毛细管电泳仪的压力系统进行亲和色谱实验。采用流动相切换洗脱技术 ,牛血清白蛋白和溶菌酶获得了有效的分离。连续运行 10次 ,溶菌酶的出峰时间、峰面积和峰高的相对标准偏差分别为0 1% ,4 3% ,3 7%。在 8 6ng～ 2 8 7ng范围内 ,溶菌酶的进样量与峰面积和峰高都呈线性关系 ,其相关系数分别为 0 9946和 0

A novel mode of affinity chromatography based on the open tubular column (OTAC) is presented in this paper The OTAC column was prepared by coating a layer of Cibacron Blue F3GA onto the inner surface of capillary column with 50 μm inner diameter The affinity chromatography experiment was performed on a capillary electrophoresis instrument by using its pressure system as driving force The bovine serum albumin (BSA) and lysozyme (Lys) were successfully separated with step gradient elution The relative standard deviation (RSD) for 10 consecutive runs for the elution time of lysozyme was less than 0 1%, and those for its peak area and peak height were 4 3% and 3 7% respectively The good linear relationships of the amount of the injected lysozyme versus its peak area and peak height were observed with correlation coefficients of \{0 994 6\} and \{0 998 8\} respectively in the range from 8 6 ng to 28 7 ng