人参皂苷正相薄层色谱二维分离的优化

Practical Optimization of the Two-Dimensional Thin-Layer Chromatographic Separation of Ginsenosides

在薄层色谱分离人参皂苷的两种互补性的流动相组分比例与皂苷比移值呈相关关系的基础上 ,以薄层色谱分离中距离最小的两点之间的距离Dmin为优化标准 ,对人参皂苷的二维分离结果进行预测和优化。通过计算机扫描不同流动相组成下的Dmin值得到的三维网络图显示出的最大Dmin下的二维流动相的组成 ,即为达到最佳二维分离结果的流动相组成。以优化的流动相对皂苷进行二维展开 ,分离结果与预测结果吻合 。

A computer assisted method is presented for optimization of mobile phase selection for separation of the mixture of ginsenosides in two dimensional TLC This method bases on the correlation between the proportions of mobile phases and the retention values R f of ginsenosides, and uses D min (the minimum distance of two spots) as the selection criterion with a two factor ( X s, Y s) statistical scanning technique The experimental results, from which we observe that more new spots are separated than on one dimensional TLC, are in excellent agreement with the predicted results.