高效液相色谱法测定磷酸苯丙哌林片的含量

Determination of Benproperine Phosphate Tablets by High Performance Liquid Chromatography

采用反相高效液相色谱法 ,以醋酸可的松为内标 ,测定磷酸苯丙哌林片的含量。色谱柱为ODS柱 ,以甲醇 水 冰醋酸 三乙胺溶液 (体积比为 6 0∶35∶5∶0 1)为流动相 ,检测波长为 2 70nm ,磷酸苯丙哌林在 9 96mg/L～ 49 8mg/L范围内有很好的线性关系 (r=0 9998) ,平均回收率为 99 91% (n =5 ) ,RSD =0 43%。

A reversed phase HPLC method for the determination of benproperine phosphate tablets is reported The chromatographic conditions were Hypersil C\-\{18\} column(5 μm, 4 6 mm i.d.×150 mm) at 35 ℃,mobile phase of methanol water glacial acetic acid triethylamine(60∶35∶5∶0 1,V/V) with a flow rate of 0 9 mL/min and UV detection at 270 nm Cortisone acetate was selected as the internal standard The linear relationship of calibration curve was good in the range of 9 96 mg/L 49 8 mg/L( r =\{0 999 8\}) The average recovery and RSD were 99 91%( n =5) and 0 43% respectively The total time of analysis for a run was within 7 min The method is simple, sensitive, rapid and accurate.