荧光探针法对N-异丙基丙烯酰胺-丙烯酰胺共聚物水溶液相变的研究

A STUDY ON THE PHASE CHANGE OF POLY(N-ISO-PROPYLACRYLAMIDE -CO-ACRYLAMIDE)AQUEOUS SOLUTION BY FLUORESCENT PROBE

合成了多种不同配比的N 异丙基丙烯酰胺 (NIPAM )与丙烯酰胺 (AM )的共聚物 .用荧光探针法研究了共聚物溶液荧光光谱随温度升高而引起的变化 .研究表明 :共聚物的最低临界溶液温度 (LCST)取决于AM与NIPAM的组成比 .AM的比例越大 .LCST就越高 ,且有较好的正比关系 .通过以共价键连接于共聚物的荧光探针法 (标记法 )和以小分子探针混入共聚物水溶液的方法 (混入法 )测定上述体系LCST的结果比较 ,发现标记探针法具有较高的灵敏度 ,更适宜用来研究共聚物水相体系的相变问题 .

A group of Poly( N \|iso\|propylacrylamide\| co \|acrylamide) with different component ratio have been prepared in this work.The change of the fluorescence spectrum of probes dissolved in the copolymer solution in water was studied with increase of temperature.The results indicated that the lower critical solution temperature (LCST) depends on the component ratio of the copolymers. The higher contents of AM in copolymers,the higher LCST was observed.By comparison of the two methods of using fluorescent probes—labeled on copolymer backbones and dissolved in aqueous solutions,it was found that the labeled probe method exhibits higher sensitivity and is more suitable for the study of the phase transition of polymer solutions.