胶束增敏紫外吸光光度法测定安宫黄体酮的研究

Study on the Determination of Medroxyprogesterone Acetate by Micelle Enhanced Ultraviolet Spectrophotometry

用紫外吸光光度法对硫酸介质中安宫黄体酮与溴代十六烷基吡啶形成的胶束配合物的性能和条件进行了系统研究 ,提出了利用胶束的增敏、增稳作用测定安宫黄体酮的紫外吸光光度新方法。本法灵敏度高 ,表观摩尔吸光系数 ε2 80 为 5 .6 10 1× 10 4L· m ol- 1· cm- 1 ,检出限为 2 .9× 10 - 8m ol· L- 1。该方法用于片剂中安宫黄体酮含量的测定 ,与药典法对照结果吻合。

In an acidic medium(pH=0.56—1.20),a new micelle enhanced ultraviolet spectrophotometry for the determination of medroxyprogesterone acetate(MA) was developed.In the presence of cetyl pyridine bromide(CPB),the maximum absorption wavelength is 280nm,and its molar absorptivity is 5.6101×10 4 L·mol -1 ·cm -1 .Beer's law is obeyed in the range of 0—7.4506×10 -6 mol·L -1 .This method was applied to the determination of MA in tablet with satisfactory results