水溶性BSA-羟基磷灰石-碳酸钙复合物的形成机理

Study on the Formation Mechanism of Soluble Complexes Containing Bovine Serum Albumin and Calcium Hydroxyapatite and Calcium Carbonate

本文以傅里叶变换红外光谱为手段 ,对不同反应摩尔比的牛血清白蛋白 (BSA)、羟基磷灰石、碳酸钙固体在水溶液中反应后的滤液进行了研究。谱带峰位和强度的变化表明 ,BSA与羟基磷灰石、碳酸钙以多种形式的络合配位及氢键等结合 ,使难溶钙盐的溶解度增加 ,形成三元水溶性复合物 ,其复合物的组成随反应摩尔比的改变而有差异 ,具有非化学计量的性质。

Fourier transform infrared spectroscopy was used to study the composition and microstructure of soluble complexes produced by different initial mole ratios of bovine serum albumin(BSA) and calcium hydroxyapatite [Ca 10 (OH) 2(PO 4) 6] and calcium carbonate (CaCO 3) in aqueous solution.The band shifts and intensity variations suggested that calcium complexes with multiple ligands such as BSA,apatite,carbonate as well as the hydrogen bond network formed in titled system.The interactions increased the solubility of Ca 10 (OH) 2(PO 4) 6 and CaCO 3 and the composition of soluble complexes is nonstoichiometric.The results provided an ideal model to investigated biomineralization process.