稀土金属离子对5’-AMP和3’,5’-cAMP的水解切断作用

Hydrolytic Cleavage of Adenosine 5'-Monophosphate and Adenosine-3',5'-Cyclic Phosphate by Rare Earth Metal Ions

用 HPL C法研究了稀土金属离子 RE3+ (如 L a3+ ,Ce3+ ,Nd3+ ,Sm3+ ,Eu3+ ,Tb3+ )对 5’-腺苷 -磷酸 (5’-AMP)和 3’,5’-腺苷环磷酸 (3’,5’- c AMP)的催化水解切断作用。结果表明 :在温和条件下 ,稀土金属离子对 5’-AMP无明显的切断作用 ,而 Ce3+ 对 3’,5’- c AMP有很好的切断作用 ;另外 ,H2 O2 的加入能极大地促进稀土金属离子对 5’- AMP和 3’,5’- c AMP的水解切断作用。 p H值对稀土金属离子水解切断 5’-

The hydrolytic cleavage of adenosine-5'-monophosphate and adenosine 3',5' cyclic phosphate by six rare earth metal ions La 3+ ,Ce 3+ ,Nd 3+ ,Sm 3+ ,Eu 3+ and Tb 3+ was investigated by HPLC.The results show that the six rare earth metal ions are inactive for hydrolysis of 5' AMP and Ce 3+ ion effectly hydrolyzes 3',5' cAMP under mild conditions.In addition,H 2O 2 can obviously promote the hydrolysis of adenosine 5' monophosphate and adenosine 3',5' cyclic phosphate by rare earth metal ions.pH has an effect on adenosine-5'-monophosphate hydrolysis by rare earth metal ions.