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聚糖联乙炔类脂仿生膜与大肠杆菌的识别作用研究 被引量:1

The Study on Recognition of Polydiacetylene Functionalized with Mannopyranoside Mimic Membrance for Escherichia Coli
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摘要 People has known that the recognition between cells and bacteria is related to the glycolipid, as a receptor on the cell surface. Diacetylene moiety in the hydrocarbon region can be cross linked by UV irradiation to form the corresponding polydiacetylene polymer. Color changes of polydiacetylenes have been known to occur in response to a variety of environmental perturbations such as heating, pH, mechanical stress or in the case of biosensor, due to the strong π π * absorption of the conjugation backbone. In this report, polydiacetylene mixed with mannopyranoside monolayer is prepared by Langmuir Blodgett film technology, E.coli K 12 adheres on the monolayer surface after gram strain. The result shows that the mannopyraoside could be recognitized by E.coli, but couldn’t be recognitized by O 111 B 4 and S.flexneri. People has known that the recognition between cells and bacteria is related to the glycolipid, as a receptor on the cell surface. Diacetylene moiety in the hydrocarbon region can be cross linked by UV irradiation to form the corresponding polydiacetylene polymer. Color changes of polydiacetylenes have been known to occur in response to a variety of environmental perturbations such as heating, pH, mechanical stress or in the case of biosensor, due to the strong π π * absorption of the conjugation backbone. In this report, polydiacetylene mixed with mannopyranoside monolayer is prepared by Langmuir Blodgett film technology, E.coli K 12 adheres on the monolayer surface after gram strain. The result shows that the mannopyraoside could be recognitized by E.coli, but couldn't be recognitized by O 111 B 4 and S.flexneri.
出处 《高等学校化学学报》 SCIE EI CAS CSCD 北大核心 2000年第11期1655-1657,共3页 Chemical Journal of Chinese Universities
基金 国家自然科学基金! (批准号 :6 96 710 0 1) 国家自然科学基金!重点项目 (批准号 :6 98310 10 )资助
关键词 糖脂 生物膜 肠道杆菌 识别作用 LB膜 检测 Glycolipid Langmuir Blodgett film E.coli Recognition
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  • 1张希,博士学位论文,1992年

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