摘要
A Gold surface was modified with N acetylcysteine to produce a self assembled monolayer and then a single layer film of rabbit IgG was immmobilized on modified gold surface covalently using carbodiimide hydrochloride(EDC) and N hydroxysuccinimide(NHS). The competitive method(Ⅰ), the sandwich method(Ⅱ) and the surplus method(Ⅲ) were developed to determine rabbit IgG or goat anti rabbit IgG, respectively. A luminescence analyzer connected with a fluorescence microscope was employed to detect the light intensity of labeled sheep anti rabbit IgG. The results of sensitivity and dynamic range were obtained as 25 mg/L and 25—600 mg/L for method(Ⅰ), 1 0 mg/L and 1—200 mg/L for method(Ⅱ) and 25 mg/L and 25—500 mg/L for method(Ⅲ), respectively.
A Gold surface was modified with N acetylcysteine to produce a self assembled monolayer and then a single layer film of rabbit IgG was immmobilized on modified gold surface covalently using carbodiimide hydrochloride(EDC) and N hydroxysuccinimide(NHS). The competitive method(Ⅰ), the sandwich method(Ⅱ) and the surplus method(Ⅲ) were developed to determine rabbit IgG or goat anti rabbit IgG, respectively. A luminescence analyzer connected with a fluorescence microscope was employed to detect the light intensity of labeled sheep anti rabbit IgG. The results of sensitivity and dynamic range were obtained as 25 mg/L and 25—600 mg/L for method(Ⅰ), 1 0 mg/L and 1—200 mg/L for method(Ⅱ) and 25 mg/L and 25—500 mg/L for method(Ⅲ), respectively.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2000年第11期1664-1666,共3页
Chemical Journal of Chinese Universities
基金
中国人民解放军"九五"医药卫生科研基金! (批准号 :98Z0 70 )资助
