不同海拔缺氧对大鼠心肌HIF-1α的影响研究

Effect of Different Geographic Altitude on Hypoxic Inducible Factor - 1α in Rats

目的:探讨不同海拔(2 500m以内)条件下对大鼠心肌的损伤作用及NO、MDA与HIF-1α的关系。方法:实验Wistar雄性大鼠18只,分为A组(海拔55m),B组(海拔1 520m)及C组(海拔2 260m)三组。在各海拔处标准饲养3日后处死取心脏,试剂盒测定低氧诱导因子(HIF-1α),丙二醛(MDA),一氧化氮(NO)。结果:C组与A组相比,HIF-1α、MDA明显增加(P<0.01),而NO明显降低(P<0.05);B组与A组相比,HIF-1α、MDA并无显著差异(P>0.05)。结论:不同海拔对大鼠心肌的影响不同,在2 260m海拔缺氧条件下,大鼠心肌确实能形成一定程度的氧化损伤,同时自由基的增加及NO的减少能在一定程度上促进HIF-1α的稳定性。

Objective：To investigate the influence role of different geographic altitude （within 2 500m） hypoxia on damage of cardiac myocyte in rats, and its relation to NO, MDA and HIF -1. Methods： 18 Wistar male rates were randomLy divided into 3 groups：control group A （55m）, group B （1 520m） and group C （2 260m）. All rates were killed after they were standard raised three days, and the activity of HIF - 1 and the concentration of MDA and NO of the hart were measured. Results：As compared with the control group A, the contents of HIF - 1 ot and MDA of heart tissue was significantly increased （P 〈0.01 and P 〈0.01） in group C. And the contents of NO of heart tissue were significantly decreased （P 〈 0.05 ） ; in group B, the contents of HIF - 1 α and MDA were not significantly deviation compared with control group A （ P 〉 0.05 ）. Conclusions： There is a significant oxidative damage in the hypoxic cardiac myocyte at 2 260m altitudes. The increase of free radical and NO level could pro- mote the stability of HIF - 1α.