依达拉奉对血管性痴呆大鼠模型学习记忆能力的影响

Effects of edaravone on learning and memory abilities and in vascular dementia rats

目的研究依达拉奉对血管性痴呆(VaD)大鼠学习记忆及海马神经元的影响。方法将受试的30例大鼠随机分为假手术组、模型组、依达拉奉治疗组共三组,将假手术组双侧颈总动脉进行分离,不阻断血流,不注射药物。模型组、依达拉奉治疗组采用"双颈总动脉永久结扎"法建立大鼠血管性痴呆(VaD)模型。在三组造模后行Morris进行水迷宫测试,同时HE染色观察海马区神经元的变化。结果 Morris进行水迷宫测试结果显示:第1天模型组逃避潜伏期(92.6±8.1 s)明显大于假手术组(71.9±5.1 s)和依达拉奉治疗组(71.2±4.9 s),差异有统计学意义(P<0.05);第2、3、4天模型组逃避潜伏期(85.9±9.8s、66.2±8.6s、68.9±7.4s)明显大于假手术组(51.9±4.9 s、38.3±4.2 s、21.1±2.9 s)和依达拉奉治疗组(52.3±3.9 s、37.5±3.9 s、20.2±2.4 s),差异有显著统计学意义(P<0.01);神经元变性凋亡明显减少。结论依达拉奉可有效改善大鼠的学习记忆能力,并通过抗自由基的作用对VaD大鼠海马区神经元起保护作用。

Objective： To investigate the effects and mechanism of edaravone on learning and memory abilities and ex- pressing of choline acetyl transferase and high affinity choline transporter in vascular dementia （VaD） rats. Methods： The subjects of 30 cases of rats were randomly divided into sham operation group, model group and edaravone treatment group. The sham operation group with bilateral carotid arteries were isolated and not block the blood flow, do not inject drugs. Vascular dementia （VaD） model was established by ＂two- vessel method＂ in model group, edaravone treatment group. Results： The three groups underwent the Morris water maze test and immunohistochemistry to detect the expression of ChaT and ChT in hippocampus. Conclusion： The first day, Morris water maze test showed that the model group escape latency （92.6 ±8. 1s） was significantly greater than the sham operation group（71.9 ±5.1 s） and edaravone treatment group（71. 2 ±4.9 s）,the differences were statistically significant（P 〈 0.05）. Edaravone can reduce neuronie apoptosis. Results： Edaravone may be effective in improving learning and memory and protecting neurons in hippocampal in rats .