幽门螺杆菌空泡毒素相关蛋白基因、空泡毒素基因与上消化道疾病的关系

The Relationship of Hp with cagA/vacA Gene to Gastroduodenal Disorders

采用 PCR法对 77例胃、十二指肠疾病患者胃粘膜 Hp cag A基因和 vac A基因的扩增以探讨cag A基因和 vac A基因型 Hp与上消化道疾病的关系 .77例 Hp感染患者中 ,60例检测出 cag A基因 ,阳性率为 77.9% ,消化性溃疡和慢性胃炎的阳性率分别为 87.8% (43/49)和 60 .7% (1 7/2 8) ,其差别有显著意义 (p<0 .0 1 ) .vac A的 2种亚型 vac A1 和 vac A2 各占 79.2 % (61 /77)和 2 0 .8% (1 6/77) .每种胃粘膜只能扩增出 vac A1 或 vac A2 的一种产物 ,其中 vac A1 在消化性溃疡和慢性胃炎中的检出率为 87.8% (43/49)和 64.3% (1 8/2 8) ,两者差别有显著性 (p<0 .0 2 ) .这些结果提示 cag A基因和 vac A1 基因型 Hp与消化性溃疡关系密切 ,这对

To study the relationship of Hp with cagA/vacA gene to the gastroduodenal disorders, PCR was used to determine the cagA/vacA genotype of Hp in 77 cases of patients with gastroduodenal disorders. CagA gene was found in 60(77.9%) of 77 cases of Hp infected patients, including peptic ulcer(43/49, 87.8%) and chronic gastritis (17/28, 60.7%). According to x 2 test, significant difference of positive rate of Hp cagA gene existed among peptic ulcer and chronic gastritis. All Hp(77/77, 100%) carried vacA gene. Either, not both, of the two subgenotypes of vacA(vacA 1 &vacA 2) was detected in an individual specimen. In the 77 cases, the positive tate of vacA 1 gene was 79.2%(61/77) and that of vacA 2 gene was 20.8%(16/77). In the 61 cases with vacA 1 gene, vacA 1 gene was detected in patients of peptic ulcer (43/49, 87.8% ), and chronic gastritis(18/28, 64.3%). x 2 test showed that the positive rate of Hp with vacA 1 gene in peptic ulcer differed significantly from that in chronic gastritis. The results indicate that Hp with cagA/vacA 1 gene is strongly related to gastroduodenal disorders, which is meaningful to prognosis and therapy of gastroduodenal disorders infected by Hp.