一株耐低温沼气促进菌的分离鉴定及最适沼气发酵条件研究

Isolation,Identification and Optimal Biogas Fermentation Conditions of a Psychro-tolerant Stain

从渭源太白山沼泽淤泥中分离筛选出一株促进沼气发酵、增加产气量的耐低温菌株xG4。通过生理、形态与16SrDNA序列的同源性分析，鉴定为原玻璃蝇节杆菌（Arthrobacter protophormiae）。该菌在15～20℃的室温条件下能使牛粪沼气发酵的产气量提高36．6％。优化了接XG4菌的牛粪沼气发酵条件，确定原料最佳初始pH值7．5，原料最佳TS质量分数为8％，菌液最佳接种量为25％。

A promoting biogas fermentation and psychro-tolerant strain XG4 was isolated from sludge collected in TaiBaishan wetland, Weiyuan. On the basis of physiology, morphology, structural characteristics and phylogenetic analysis,it was turned out that XG4strain was a species of the Arthrobacter protophormiae. The cow dung biogas production was increased by 36.6% after inoculating the stain of XG4 at fifteen to twenty temperature grades. The optimum biogas fermentation conditions for XG4 were as follows ： the initial pH was 7.5, the mass fraction of total solid was 8% , the inoculation was 25%.