麝香百合离体繁殖与移栽技术研究

STUDIES ON THE TECHNOLOGY OF PROPAGATION IN VITRO AND TRANSPLANTATION

对麝香百合(LiliumlongiflorumThunb)在离体快速繁殖中的取材部位、生根培养基中的激素配比和移栽基质进行了研究,结果表明:幼茎段和中部鳞片是较优材料;在生根培养中,培养基以 1/2MS +NAA 0.5mg/l + 2 %蔗糖 + 0.7%琼脂(pH5.7)为宜;炼苗 3天后生根幼苗转入移栽介质,最适宜过渡基质为 1/3园土 + 1/3腐殖土 + 1/3锯末,过渡 10天后进入大田。过渡基质对麝香百合移栽大田后的成活率没有影响,但对其地上、地下部分的营养生长有显著影响。

The suitable explants, the ratio of BA to NAA in the root-initiation medium and the transplant medium are studied. The optimum explants are immature stem segments and bulb scales. The root initiation is enhanced when the culture medium is 1/2 MS+NAA 0.5mg/l+2% Sucrose+0.7% agar(pH5.7). After three days acclimatization, the plantlets with roots should be cultivated in the transplant medium prepared with ordinary soils, decayed loam and saw dust (1∶1∶1). Ten days later, these plantlets are planted in the field. Although the surrival rate is not affected by the transplant composition, the vegetative growth of the plantlet varies obviously, either the first leave length or the root length, number and bulb size.