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Cellular Reprogramming of Human Peripheral Blood Cells 被引量：2

Cellular Reprogramming of Human Peripheral Blood Cells
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摘要 Breakthroughs in cell fate conversion have made it possible to generate large quantities of patient-specific cells for regenerative medicine. Due to multiple advantages of peripheral blood cells over fibroblasts from skin biopsy, the use of blood mononuclear cells （MNCs） instead of skin fibroblasts will expedite reprogramming research and broaden the application of reprogramming technology. This review discusses current progress and challenges of generating induced pluripotent stem cells （iPSCs） from peripheral blood MNCs and of in vitro and in vivo conversion of blood cells into cells of therapeutic value, such as mesenchymal stem cells, neural cells and hepatocytes. An optimized design of lentiviral vectors is necessary to achieve high reprogramming efficiency of peripheral blood cells. More recently, non-integrating vectors such as Sendai virus and episomal vectors have been successfully employed in generating integration-free iPSCs and somatic stem cells. Breakthroughs in cell fate conversion have made it possible to generate large quantities of patient-specific cells for regenerative medicine. Due to multiple advantages of peripheral blood cells over fibroblasts from skin biopsy, the use of blood mononuclear cells （MNCs） instead of skin fibroblasts will expedite reprogramming research and broaden the application of reprogramming technology. This review discusses current progress and challenges of generating induced pluripotent stem cells （iPSCs） from peripheral blood MNCs and of in vitro and in vivo conversion of blood cells into cells of therapeutic value, such as mesenchymal stem cells, neural cells and hepatocytes. An optimized design of lentiviral vectors is necessary to achieve high reprogramming efficiency of peripheral blood cells. More recently, non-integrating vectors such as Sendai virus and episomal vectors have been successfully employed in generating integration-free iPSCs and somatic stem cells.
作者 Xiao-Bing Zhang
机构地区 Department of Medicine
出处 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2013年第5期264-274,共11页 基因组蛋白质组与生物信息学报（英文版）
基金 supported by the Grants for Research and School Partnerships (GRASP) Award from the Loma Linda University and U.S.Army Medical Research Acquisition Activity (USAMRAA) Concept Award(Grant No.W81XWH-11-1-0607)
关键词 REPROGRAMMING Peripheral blood Hematopoietic cells Induced pluripotent stemcells Cell fate conversion Reprogramming Peripheral blood Hematopoietic cells Induced pluripotent stemcells Cell fate conversion
分类号 R329 [医药卫生—人体解剖和组织胚胎学]
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共引文献108

同被引文献53

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Genomics, Proteomics & Bioinformatics
2013年 第5期

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