全盘自动化高效液相色谱仪硬件的研制

A Study on the Hardware for a Fully Automated High-performance Liquid Chromatography System

本文报道了全盘自动化高效液相色谱仪硬件的研制工作。该仪器把梯度洗脱装置、光二极管列阵扫描检测器及自动进样器作为一个系统用一台微机进行控制、数据采集和处理,从而实现液相色谱分析的全盘自动化。实验表明,所有部件及接口电路设计合理,工作可靠,实现了整个系统的闭环控制和反馈。研制的光二极管列阵扫描检测器具有扫描速度快、光谱范围宽和灵活度高的特点。该研究为智能色谱的发展提供了可靠的物质保证。

A hardware system for a fuliy automated high-performance liquid chromatography system has been established. In order to realize full automation of chromatographic analysis, the gradient elution system with two high pressure pumps, fast-scanning photodiode array detector(PAD) and autosampler have been controlled as a system Ly IBM PC/XT microcomputer. It is shown experimentally that all units and interface circuits were reliable and reasonable. A closed-loop control and feedback of the whole system have been achieved. The PAD is charactered by fast scanning speed, high sensitivity and wide linearity. The research can support further development of chromatography with artificial intelligence.