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BMP-7在牙龈蛋白酶诱导成骨细胞凋亡中的保护作用 被引量：2

Protective Effects of BMP-7 on Osteoblasts Apoptosis Induced by gingipains
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摘要 目的:研究牙龈蛋白酶对成骨细胞MC3T3-E1的增殖及凋亡的作用,以及BMP-7在此过程中的保护作用。方法:分离提纯牙龈蛋白酶,建立牙龈蛋白酶诱导成骨细胞凋亡模型,使用MTT法、TUNEL/DAPI染色法检测牙龈蛋白酶对成骨细胞增殖及凋亡的影响,并且研究BMP-7对于牙龈蛋白酶造成的细胞凋亡的保护作用。结果:牙龈蛋白酶呈剂量和时间依赖性地抑制成骨细胞增殖,诱导成骨细胞凋亡。在500和1000 ng/ml浓度范围内,BMP-7呈剂量依赖性地减轻牙龈蛋白酶对成骨细胞的增殖抑制及凋亡诱导作用。结论:BMP-7能有效抵抗牙龈蛋白酶诱导产生的成骨细胞增殖抑制及细胞凋亡。 Objective： The aim of this study was to determine the proliferative and apoptotic effects of gingipains on os- teoblasts, and to study the protective effects of BMP-7 on apoptosis induced by gingipains. Methods： Gingipains from por- phyromonas gingivalis W83 was purified and MTT assay and TUNEL/DAPI staining were used to determine the prolifera- tive and apoptotic effects of gingipains and BMP-7 on MC3T3-E1. Results： Gingipains inhibited prolifbration and en- hanced apoptosis ofosteoblasts in a dose and time dependent manner. At certain concentrations （500ng/ml and 1000ng/ml）, BMP-7 protected the osteoblasts by promoting proliferation and reducing apoptosis. Conclusions： BMP-7 effectively res- cued gingipains-induced proliferative inhibition and aDontosis ofMC3T-EI
作者 郭晓莹 朱文俊 宋祥晨 付云 梁敏
机构地区 中山大学附属口腔医院 中山大学附属口腔医院牙周科
出处 《中华老年口腔医学杂志》 2013年第3期140-144,共5页 Chinese Journal of Geriatric Dentistry
基金 国家自然科学基金(编号:81170970) 留学归国人员科研启动基金(编号:2009134) 广东省科技计划(编号:2011 B031800259)
关键词 牙龈蛋白酶 成骨细胞 细胞凋亡 BMP-7 gingipains, osteoblasts, apoptosis, BMP-7
分类号 R781.4 [医药卫生—口腔医学]
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参考文献12

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二级参考文献42

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共引文献254

同被引文献33

  • 1贝抗胜,李康华,吴强,刘建平,钟延东,杨献群,李雄,熊英辉.人骨膜细胞体外培养的实验研究与临床应用[J].中国矫形外科杂志,2004,12(17):1329-1331. 被引量：4
  • 2温学红,郭若霖,程瑞芳,张福江,谈志龙,叶伟胜,王学谦.BMP-2和地塞米松对成人成骨细胞增殖和分化的影响[J].临床检验杂志,2005,23(1):34-37. 被引量：5
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引证文献2

二级引证文献4

中华老年口腔医学杂志
2013年 第3期

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