火菇素纯化流程的改进

IMPROVEMENT OF PURIFYING PROCEDURE OF FLAMMULIN

利用前文建立的火菇素免疫单向琼脂扩散定量检测技术 ,对火菇素纯化流程作了定量监测 ,针对一、二、三等金针菇原料的不同特点 ,对纯化技术作了相应改进 ,可分别使火菇素的提取率提高 71 .3% ,1 87.6%和 2 1 7.9% ,1Kg风干子实体的火菇素产量达到 1 0 9.1mg ,52 .3mg和 2 7 0mg .

With the help of the measurement method established according to simple agar diffusion of flammulin with antiserum on agarose gel plate (see the former article),flammulin in each purifying step of the primary procedure was detected.A new purifying procedure according to different kinds of raw materials of basidioma (Grade I,Ⅱ and Ⅲ) of Flammulina velutipes was established,which provided a higher extracting rate and the flammulin production increased significantly by 71.3%,187.6% and 217.9%,reaching 109.1 mg,52.3 mg and 27.0 mg,respectively.