石榴皮鞣质对慢性肾小球肾炎的改善作用

Improving effect of tannins in Pomegranate rind on chronic glomerulonephritis

目的:研究石榴皮鞣质对慢性肾小球肾炎的改善作用。方法:采用大鼠尾静脉注射阳离子化牛血清白蛋白(cBSA)建立慢性肾小球肾炎(CGN)模型,造模成功后,石榴皮鞣质样液灌胃4周。分别收集大鼠在造模后和给药后1,2,3,4周的24 h尿液和血液,进行尿蛋白、血清肌酐和尿素氮的检测;实验结束后,摘取大鼠双侧肾脏,观察肾组织结构形态。结果:检测结果显示,与模型组相比,石榴皮鞣质高剂量组、低剂量组的尿蛋白含量均有所降低,血清肌酐和尿素氮的含量也呈下降的趋势,病理学结果显示,石榴皮鞣质给药组大鼠的肾组织学结构形态较为正常,与模型组比较仅有少部分损伤。结论:石榴皮鞣质能够降低CGN大鼠尿蛋白,血清肌酐和尿素氮的含量,改善其肾组织结构形态,对慢性肾小球肾炎具有一定的改善作用。

ORIECTIVE To study the improving effect of tannins in Pomegranate rind on chronic glomerulonephritis, and to explore its mechanism of action. METItODS Cationized bovine serum albumin （cBSA） injection was injected into the tail vein of rats for establishing the model of chronic glomerulonephritis. The rats were randomly divided into 5 groups： normal control group, model group, high dose group, low close group and the positive control group; then the drugs were given orally for 4 weeks. The urine of 24-hours and the blood of rats were collected after modeling and at 1,2,3,4 week after treatment, the u- rine protein in 24-hours, serum creatinine and urea nitrogen were determined. At the end of the experiment, the kidney of the rats were removed out for observing the changes of renal histological morphology. RESULTS The results showed that the con tents of 24-hours urine protein of the high-dose group and low dose group were decreased compared with the model group. Cor- respondingly, the contents of serum creatinine and urea nitrogen also showed a downward tendency. The pathological results demonstrated that the renal histological morphology of the drug treatment groups was relatively normal, only a small part of damage, as compared with the model group. CONCLUSION Tannins in Pomegranate rind played a significant role in reducing urinary protein,serum ereatinine and urea nitrogen,and in improving the structure of kidney, which showed that the tannins in Pomegranate rind had some improving effects on chronic glomerulonephritis.