摘要
目的 观察周期性应力下磷脂酶C-γ1(PLCγ1)及其抑制剂对大鼠髓核细胞增殖的影响.方法 将细胞玻片随机分成4组:对照组、加压0.5h组、加压1h组、加压2h组.Western blot法检测各组PLCγ1、磷酸化PLCγ1表达.另取细胞玻片,随机分为3组:对照组、加压6h组、U73122+加压6h组.使用荧光实时定量聚合酶链反应(FQ-PCR)检测各组细胞外基质的表达.结果 PLCγ1磷酸化水平在加压0.5h组(0.109 ±0.010)、加压1h组(0.138±0.001)、加压2h组(0.201 ±0.014)较对照组(0.086 ±0.005)增高.加入U73122后,细胞外基质的基因表达,以对照组最低,加压6h组最高,U73122+加压6h组居中,差异有统计学意义(P<0.05).结论 周期性压力能促进髓核细胞的增殖,PLCγ1在压力传导中起信号传递作用.
Objective To evaluate the effect of the phospholipase C-γ1 (PLCγ1) protein and its inhibitor on rat nucleus pulposus cells proliferation under periodic mechanical stress.Methods Some cell slides were randomized into four groups:the control group,0.5 h stress group,1 h stress group and 2 h stress group.The expression of PLCγ1 and pPLCγ1 was detected by using Western blotting.The other slides were randomized into three groups:the control group,6 h stress group and U73122 + 6 h stress group.Type Ⅱ collagen and aggrecan gene expression levels were evaluated through real-time fluorescent quantitative polymerase-chain reaction.Results The pPLCγ1/PLCγ1 ratio in 0.5 h stress group (0.109 ± 0.010),1 h stress group (0.138 ±0.001) and 2 h stress group (0.201 ±0.014) was significantly higher than in control group (0.086 ± 0.005).The gene expression levels of type Ⅱ collagen and aggrecan in U73122 + 6 h stress group were significantly higher than those in control group,but lower than those in 6 h stress group (P 〈 0.05).Conclusion Periodic mechanical stress can promote the proliferation of rat nucleus pulposus cells.PLCγ1 plays important roles in the mechanical transductions.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第11期2361-2363,共3页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金资助项目(81201417)
江苏大学医学临床科技发展基金项目(JLY20120079)
关键词
磷脂酶C-Γ1
周期性应力
髓核细胞
Keywords:Phospholipase C-γ1 protein
Periodic mechanical stress
Nucleus pulposus cell