红花黄色素对大鼠皮瓣再灌注损伤的影响

Effects of Saffloryellow on survival rate of skin flaps with ischemia-reperfusion injury in rats

目的 观察红花黄色素对大鼠腹部皮瓣缺血再灌注损伤的影响,探讨其机制.方法 32只SD大鼠建立腹壁轴形缺血再灌注损伤皮瓣模型,随机平均分为两组,实验组再灌注前1h及术后6d内每日腹腔注射红花黄色素[40 mg/（kg·d）],对照组给予等剂量生理盐水.再灌注24 h测皮瓣内超氧化物歧化酶（SOD）、丙二醛（MDA）含量；术后第7天,观察皮瓣成活率、皮瓣组织学变化及多形核白细胞（PMN）计数.结果 相对于对照组,实验组皮瓣内SOD水平升高而MDA及PMN水平则明显降低（P＜0.01）；术后第7天皮瓣成活率,实验组[（66.53±7.86）％]高于对照组[（38.17±8.29）％],差异有统计学意义（P＜0.01）.结论 红花黄色素可明显减轻再灌注损伤,促进皮瓣成活.作用机制与其能拮抗血小板活化因子,减少中性粒细胞和血小板聚集,提高SOD活性,清除氧自由基,减轻脂质过氧化反应有关.

Objective To investigate the effects of Saffloryellow （SY） on the flaps with ischemiareperfusion injury （IRI） in rats and the mechanism.Methods Thirty-two SD rats were used to establish IRI skin flaps along superficial epgastric artery.All rats were randomly divided into the control and experimental groups （n =16 each）.SY injection [40 mg/（kg· d）] and normal saline with the same volume were injected into the abdominal cavity of rats respectively at 1 h prior to the reperfusion and the next 6 days after operation.The survival rate,pathologic changes,levels of superoxide dismutase （SOD）,malondialdehyde （MDA） and polymerphonuclear leucocytes （PMN） of the flaps were analyzed.Results The contents of MDA and PMN were decreased （P 〈0.01）,while the activity of SOD increased in the experimental group （P 〈 0.01）.The mean survival rate of the flaps at the 7th day after operation was （38.17 ±8.29） % in the control group and （66.53 ± 7.86） % in the experimental group （P 〈 0.01）.Conclusion SY can reduce the IRI and increase the survival rate of the flaps significantly in rats probably by inhibiting the activation of PAF and PMN,declining the blood viscosity,improving the activity of SOD,reducing the level of oxygenderived free radical and lipid peroxidation.