胃癌患者血清糖蛋白Dickkopf-1水平检测及手术前后血清糖蛋白Dickkopf-1水平变化

Changes of serum dickkopf-1 before and after operation in patients with gastric carcinoma

目的 探讨胃癌患者血清糖蛋白Dickkopf-1表达及手术前后血清糖蛋白Dickkopf-1表达水平变化的临床价值.方法 采用酶联免疫吸附试验（ELISA）法检测32例正常人以及64例胃癌患者血清Dickkopf-1水平,并比较41例胃癌患者手术前后血清Dickkopf-1水平.结果 胃癌患者血清Dickkopf-1水平[（7.95 ±1.50） μg/L]明显高于正常对照组[（3.23±0.60）μg/L],两者比较差异有统计学意义（P＜0.01）；血清Dickkopf-1水平与胃癌患者性别、年龄不相关,与胃癌患者临床分期、细胞分化程度有相关；Ⅳ期[（9.72±0.56） μg/L]明显高于Ⅲ期[（8.61±0.61） μg,/L]、Ⅲ期[（8.61±0.61） μg/L]明显高于Ⅱ期[（7.58±0.52） μg,/L]、Ⅱ期[（7.58 ±0.52） μg/L]明显高于Ⅰ期[（5.67±0.63） μg/L]、Ⅰ期[（5.67±0.63） μg/L]明显高于正常对照组[（3.23±0.60） μg/L],且两两比较差异有统计学意义（P＜0.01）,高分化胃癌患者血清Dickkopb1水平[（7.03±1.45）μg/L]和中分化胃癌患者血清Dickkopf-1水平[（7.96±1.51） μg/L]低于低分化胃癌患者[（8.82±0.98） μg/L]（P＜0.05）；手术后胃癌患者血清Dickkopf-1水平[（6.89±1.27） μg/L]明显低于手术前[（7.19±1.26） μg/L],两者比较差异有统计学意义（P＜0.01）.结论 Dickkopf-1可能参与胃癌的发生发展过程,能够反映疾病进展的情况.

Objective To investigate the changes of serum Dickkopf-1 before and after operation in patients with gastric carcinoma and its relationship with clinicopathological characteristics. Methods Enzyme linked immunosorbent assay （ELISA） was used to detect the level of serum Dickkopf-1 in 64 cases of patients with gastric carcinoma and 32 cases of normal controls, and the changes of serum Dickkopf-1 before and after operation in 41 cases of gastric carcinoma were compared. Results The level of serum Dickkopf-1 was elevated in all subgroups of patients with gastric carcinoma [ （7.95 ± 1.50） μg/L] compared to the con- trols [ （3.23±0. 60） μg/L] （P 〈0. 01 ）. Serum Dickkopf-1 was correlated with TNM stage and histological grade, but not with sex and age. The serum Dickkopf-1 level in stage Ⅳ [ （9. 72 ±0. 56） μg/L] was higher than in stage Ⅲ [ （8.61 ±0. 61） μg/L] （P 〈0. 01）, that in stage Ⅲwas higher than in stage Ⅱ [ （7.58 ±0.52） μg/L] （P〈O. 01）, that in stage Ⅱwas higher than that in stage I [（5.67 ±0.63）μg/L] （P〈 0. 01）, and that in stage I was higher than that in normal controls [ （3.23 ± 0. 60） μg/L] （P 〈0. 05）. The serum Dickkopf-1 level was lower in the well differentiated [ （7.03 ± 1.45） μg/L] and moderately differenti- ated [ （7. 96 ± 1.51 ）μg/L] than the poorly differentiated gastric carcinoma [ （8. 82 ±0. 98） μg/L] （P 〈 0. 05）. The serum Dickkopf-1 levels were remarkably reduced in patients with gastric carcinoma after opera- tion （P 〈 0. 01 ）. Conclusion Dickkopf-1 may take part in the malignant process of gastric carcinoma, and can be used as the disease indicators for clinical monitorings.