青海大黄油菜粒色性状AFLP标记的筛选和SCAR标记转化

AFLP and SCAR markers for seed colour in rape Dahuang(Brassica rapa L.)

大黄油菜是青藏高原特有的一个白菜型油菜地方品种,种皮颜色鲜黄。已知大黄油菜的黄籽性状受到1对隐性基因(Brsc1)控制,且该基因被定位于白菜型油菜的第9连锁群上。为获得更多与种皮色泽紧密连锁的分子标记以及共显性标记,本研究利用大黄油菜和褐籽白菜型油菜09A-126为亲本构建BC1分离群体和F2群体,利用AFLP与BSA相结合的方法,通过筛选256对引物结合,共获得5个与种皮色泽连锁的AFLP标记。5个特异AFLP片段分别被回收、克隆、测序,并与白菜型油菜基因组序列进行同源比对,均与A09染色体表现同源。将5个AFLP标记成功转化成5个SCAR标记,用F2群体对SCAR标记进行检测,筛选到1个共显性标记。

Rape Dahuang is a special landrace of Brassica rapa L.which originated from Qinghai-Tibetan Plateau with bright yellow seeds.Previous results showed the yellow-seeded trait in Dahuang was controlled by a recessive gene （Brsc1）,which was mapped on A09 in B.rapa.For more specific markers linked to Brsc1,BC1 population and F2 population were constructed from the cross of Dahuang and 09A-126 （brown-seeded,B.rapa）.Amplified fragment length polymorphism （AFLP） technology combined with bulked segregant analysis （BSA） was conducted by screening 256 pairs of AFLP primers in BC1 population,5 markers tightly linked to Brsc1 were obtained.5 specific AFLP fragments were cloned,sequenced and identified,and compared to that of synteny in B.rapa genome,showing homology to A09 of B.rapa.The AFLP markers were converted into 5 SCAR （sequence characterized amplified region） markers for F2 population.One co-dominant marker was detected.