11个红花品种遗传多样性与亲缘关系的SRAP分析

Analysis of genetic diversity and relationship of 11 Carthamus tinctorius L.varieties by SRAP

利用SRAP技术对来源于中国不同地区的11份红花品种材料亲缘关系进行了分析.选用了25对具有多态性条带的引物组合,获得了308条清晰的条带,其中109条具有多态性,多态性位点百分率为35％.遗传相似系数变幅在0.82 ～ 0.934 2之间,平均相似系数为0.87,表明红花种内不同品种间存在一定的遗传多样性.聚类结果分析表明,在相似系数GS=0.891 4处,可以将11份红花材料分为四类.

11 Carthamus tinctorius L.varieties were used to study their genetic diversity and relationship using SRAP.With 25 SRAP primers,308 bands were amplified,among them 109 bands （35％） were polymorphism.The genetic similarity coefficients ranged from 0.82 to 0.934 2 with an average of 0.87,indicating the existence of genetic diversity among these 11 varieties which were divided into four groups by cluster analysis at the similarity coefficient of 0.891 4.