油菜干旱相关基因的表达及其与耐旱生理指标的相关性

Correlation between drought-related genes expression and physiological indicators in rapeseed

选取8个不同抗旱性油菜材料,研究6种干旱相关基因(btg-26,BnD22,P5CS,CaM,BADH,ADC)在不同胁迫持续时间下的表达情况,并结合5个耐旱生理指标以探究油菜中干旱相关基因的表达与耐旱生理指标之间的相关性。结果表明,btg-26,BnD22,P5CS,BADH,ADC在抗旱材料Q2和敏感材料Zs6和S95-3中表达模式差异明显。5个生理指标中,叶片萎蔫指数和相对电渗率的变异程度最大。相关性分析表明,btg-26在干旱胁迫24h的表达量与相对电渗率显著相关,与相对含水量极显著相关。而胁迫3h,P5CS和ADC的表达量与叶片萎蔫指数和相对电渗率显著相关,BADH的表达量与相对电渗率之间显著相关。在考察油菜耐旱性时,这些基因的表达情况可以作为抗旱性鉴定的参考指标。

Correlation between gene expressions and physiologies was investigated under drought stress using 8 tolerant and susceptive rapeseed materials.The genes included 6 drought-related genes （btg-26,BnD22,P5CS,CaM,BADH and ADC）.Results showed that the expression profiles were different among the genes between tolerant rape Q2 and susceptive materials Zs6 and S95-3.Among the 5 indicators,leaf wilting index （LWI） and relative electrolyte leakage （REL） varied largely among the varieties under drought.Correlation analysis showed that after 3h of drought,P5CS was significantly correlated to LWI and REL,BADH was correlated with REL.After 24h of drought,big-26 was significantly correlated with relative water content and REL.Results indicated that the above genes could be appraisal indices of drought-tolerance.