乳酸亚铁对两种致病菌的生长抑制及细胞膜通透性的影响

Growth Inhibition and Cell Membrane Damage of Two Foodborne Pathogens by Ferrous Lactate

以金黄色葡萄球菌和铜绿假单胞杆菌为供试菌,通过测定其生长曲线和胞内物质外泄情况,研究乳酸亚铁溶液对这两种具有代表性的食品致病菌的生长抑制及细胞膜通透性的影响。结果表明:经过乳酸亚铁溶液处理后,菌体的生长受到抑制,并未达到正常高峰;与对照组相比,乳酸亚铁溶液处理组细菌培养液的电导率增加>14%,菌液的OD260nm增加>42%,培养液中还原糖含量增加>40%,可溶性蛋白质含量增加>6.87%。由此表明,乳酸亚铁溶液能够增大菌体细胞膜的通透性,导致胞内物质大量泄漏,从而抑制菌体的生长。

The effect of ferrous lactate on growth inhibition and cell membrane damage of Staphylococcus aureus （G＋）and Pseudomonas aeruginosa （G－） were investigated through the determination of growth curve and leakage of intracellularsubstances. As the results showed, the growth of the pathogenic bacteria was inhibited after ferrous lactate treatment,accompanied by increases in the conductivity, OD260nm, reducing sugar content and soluble sugar content by 14%, 42%, 40%and 6.87%, respectively, as compared to the control group. These observations indicated that ferrous lactate solution coulddamage cell membrane, thus resulting in an increase in the permeability of cell membrane and the release of large amountsof intracellular contents, and finally inhibiting bacterial growth.