阿魏酸对酒类酒球菌生长作用机理的研究

Mechanism Underlying the Effect of Ferulic Acid on the Growth of Oenococcus oeni

以酒类酒球菌质膜蛋白含量和H+-ATPase活性为研究指标,探索阿魏酸对酒类酒球菌生长的作用机理。结果表明:阿魏酸能够通过破坏酒类酒球菌细胞膜结构抑制生长,主要表现在质膜蛋白含量的升高和H+-ATPase活性的降低。50mg/L阿魏酸能够强烈抑制酒类酒球菌的生长,并且随阿魏酸含量的增加抑制作用逐渐增强。不同菌株受到阿魏酸的抑制程度有所不同,在选择的3株供试菌株中,O.oeniHB-2b受到的抑制作用最弱,O.oeniSD-2a和O.oeniSD-2gf的抑制程度相对较强。

The study aimed to explore the mechanism behind the effect of ferulic acid on the growth of Oenococcus oenithrough determining plasma membrane protein content and H＋-ATPase activity. The results showed that ferulic acid wasable to inhibit microbial growth by destroying the cell membrane structure of Oenococcus oeni, especially elevating plasmamembrane protein content and reducing H＋-ATPase activity. Ferulic acid at 50 mg/L strongly suppressed the growth ofOenococcus oeni in a dose-dependent manner. Different strains made varying responses to different levels of ferulic acid.The inhibition effect of ferulic acid on the three strains tested decreased in the following order： O. oeni SD-2a ＞ O. oeniSD-2gf ＞ O. oeni HB-2b.