存活素在妊娠滋养细胞肿瘤中的表达及其与Fas/Fas-L表达的相关性研究

Expressions of Survivin and relationship with Fas/Fas-L expression in gestational trophoblastic tumor

目的探讨存活素(Survivin)在妊娠滋养细胞肿瘤(GTT)中的表达及其与Fas/Fas-L表达的相关性。方法应用免疫组织化学方法检测存活素在侵蚀性葡萄胎(15例)及绒毛膜上皮癌(绒癌)(11例)中的表达,正常滋养细胞(40份)作为对照;并与Fas/Fas-L表达进行相关性分析。结果存活素在15例侵蚀性葡萄胎组有9例(60%)阳性,在11例绒癌组有8例(73%)阳性。而在40份正常滋养细胞中仅有11例(28%)阳性,存活素在GTT中的表达增高,且随着妊娠滋养细胞疾病的进展阳性率逐渐增高。正常滋养细胞组与绒癌组差异有统计学意义(P<0.05)。存活素与Fas表达在绒癌中呈负相关。结论凋亡抑制蛋白存活素表达上调可能参与了GTT的发生发展。Fas表达的下调和存活素表达的上调可能在GTT的发生发展过程中起协同作用,二者共同抑制细胞凋亡,逃避免疫清除,进而导致肿瘤的发生。

Objective To determine the expressions of Survivin and its relationship with the expression of Fas/Fas-L in gestational trophoblastic tumor （GTT）.Methods Immunohistochemical assay was employed to detect Survivin expression in the tissues derived from 15 patients with invasive hydatidiform mole and 11 with choriocarcinoma,with 40 cases who had normal villi as the control group.This entailed the correlation analysis on Fas/Fas-L.Results Survivin immunoreactivity was shown in 9 of 15 cases （60％） with invasive hydatidiform mole,8 of 11 cases （72.7％）with choriocarcinoma and in 11 of 40 cases （28％） with normal villi.The expression of Survivin in GTT was heightened compared with that in the normal villi and increased progressively with the advancement of disease severity.There was a significant difference in the expression of Survivin between normal villi and choriocarcinoma group （P＜0.05）.The Fas was negatively correlated with Survivin expression in patients with choriocarcinoma.Conclusion The up-regulation of Survivin could have involved in the pathogenesis of GTT,and may act synergistically with the downregulation of Fas expression,which inhibit cell apoptosis and suppress immune clearance,thus leading to the tumorigenesis.