转基因技术及骨髓间充质干细胞在骨缺损修复中的应用与展望

Application and Prospect of Genetically Modified Technology and Bone Marrow Mesenchymal Stem Cells in the Bone Defect Repair

骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)是一类存在骨髓内的非造血干细胞,具有较强的增殖功能及多向分化潜能。在一些细胞因子的诱导下可以向成骨系细胞、内皮细胞、成纤维系细胞、成软骨系细胞等方向分化,近年来已成为生物学和医学的研究热点。而转基因技术是一项目前较为热门的生物科技技术。本文简要介绍了不同基因如骨形态发生蛋白(bone morphogenetic protein,BMP)、血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)、软骨源性形态发生蛋白1(cartilage-derived morphogenetic protein 1,CDMP-1)、碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)、成骨生长肽(osteogenic growth peptide,OGP)等通过转染后对BMSCs增殖、分化的影响及其在骨缺损修复中的应用。

Bone mesenchymal stem cell is a kind of hematopoietic stem cell within the bone marrow, with strong proliferation capability and multi-directional differentiation potential. Under some ytokines induced, it can become osteogenesis cell, endothelial cell, fibroblast cell, and cartilage cell. Bone mesenchymal stem cell has become a hotspot of biology and medicine in recent years; and genetically modified technology is a relatively popular biological technology project. This paper briefly introduces the different genes such as bone morphogenetic protein （BMP）, vascular endothelial growth factor（VEGF）, cartilage-derived morphogenetic protein 1 （CDMP-1）, basic fibroblast growth factor （bFGF）, and osteogenic growth peptide （OGP） after transfection effect on BMSC proliferation and differentiation, as well as its application in bone defect repair.