肺纤维化(气虚血瘀证)大鼠模型造模方法新探索

Exploring a new method for establishing model of pulmonary fibrosis(syndrome of Qi deficiency and blood stasis) in rats

[目的]探索内窥镜引导下制作肺纤维化(气虚血瘀证)大鼠模型的新方法。[方法]1)SD大鼠60只,运用内窥镜摄录监视系统及自创的鼠麻醉喉镜,采用无水乙醚吸入法麻醉方式,对大鼠气管内推注药物博莱霉素造肺纤维化模型。2)造模后随机分正常对照组,模型组,芪术合剂组,泼尼松组观察。中药芪术合剂组疗效显著,从以方测证的角度验证肺纤维化(气虚血瘀证)大鼠模型的制作成功。[结果]1)此造模方法具有气管内推注药物靶点准确,成功率高,无外源创伤,保障实验数据的原始性、可靠性,节约时间,提高工作效率,减少标本耗损量,降低实验成本。2)益气活血散结法中药干预以方测证结果表明,该方法制作的模型具有气虚血瘀证的病机特点。[结论]该方法的创新值得在肺系疾病大鼠模型制造实验中推广运用。

[Objective] To Explore a new method to establish a model of pulmonary fibrosis with Qi deficiency and blood stasis in rats guided with endoscope. [Methods] 1） Using the endoscopic videography monitoring system and self-made anaesthetic laryngoscope for rats, 60 SD rats were anaesthetized by anhydrous diethyl ether inhalation, and intratraeheal infused Bleomyein was performed to establish the mode of pulmonary fibrosis. 2） All the rats were divided into 4 groups randomly： normal control group, model group, Qizhu Heji group and prednison group. It was found that Qizhu Heji group had a significant effect and so the model proved to be sueeessfully established from the point of deteeting disease by prescription. [Results] 1） This modeling method has employed a method of intratraeheal infusion, which has accurate target, high success rate, no exogenous trauma, and can also ensure the original and reliable experimental data. Besides, it can be time-saving, improve work efficiency, decrease sample consumption, consequently reduce experiment cost. 2） Based on detecting disease by prescription of tonifying Qi, activating blood and removing stasis, the model shows the pathogenesis eharacteristies of Qi deficiency and blood stasis. [Conclusion] This innovative method deserves promotion in establishing model with pulmonary diseases in rats.