金属硫蛋白对铬染毒小鼠肝脏氧化损伤的修复作用

Epairment effect of Metallothionein on oxidative damage in mice liver infected with chromium

目的:探讨金属硫蛋白(MT)对六价铬(Cr6+)染毒小鼠肝脏氧化损伤的修复作用。方法:60只清洁级昆明(KM)种小鼠雌雄各半,随机分为5组:对照组,铬(Cr6+)染毒组(50 mg/kg),低、中、高(5.0、10.0、20.0 mg/kg)剂量MT保护组。对照组灌胃生理盐水,铬染毒组按50 mg/(kg·bw)灌胃重铬酸钾溶液;MT保护组在给予铬染毒的同时继续分别按5.0、10.0、20.0 mg/(kg·bw)剂量灌胃MT。各组灌胃时间均为15 d,每日1次;灌胃体积均为0.1 ml/(10g·bw)。实验结束麻醉处死动物采血,取肝脏计算其脏器系数;全自动生化分析仪检测肝功能AST、ALT、GGT含量;试剂盒检测肝组织SOD活性和MDA含量。结果:与对照组比较,铬染毒小鼠体重降低、肝脏器系数增高、血清AST、ALT、GGT增高、SOD活力下降、MDA含量增高(P<0.05)。经MT保护后与铬染毒组比较小鼠体重有所回升、肝脏脏器系数下降、血清AST、ALT、GGT降低、SOD活力升高、MDA含量下降,其恢复程度与MT呈剂量—效应关系(P<0.05)。结论:铬(Cr6+)对小鼠肝脏有损伤作用,MT对肝脏有保护作用,其机制与抗氧化作用有关。

Objective： To investigate the repairment effect of metallothionein （MT） on oxidative damage of mice liver poisoned by hexavalent chromium（Cr6^＋ ）. Methord： 30 male and 30 female clean level Kunming（KM） mice were randomly devided into 5 groups： control group, Cr6^＋ infected group, low dose of MT protection group, middle dose of MT protection group and high dose of MT protection group. The mice in control group were intragastric saline. The mice in Cr6^＋ infected group were intragastric potassium dichromate solution with 50mg/（kg· bw）, and mice in MT protection of chromium infected groups, respectively, at the same time continue to intragastric MT at the dose of 5.0, 10.0, 20.0 mg/（kg·bw） after infecting with Cr6＋ . All of the mice were treated once a day for 15 days and with gastric volume of 0. lml/（10g·bw）. At the end of the experiment, animals were given anesthesia and taken blood, then the liver organ coefficient were calculated. The liver function, AST, ALT and GGT content were detected by automatic biochemical analyzer, and SOD activity and MDA content in liver tissue were measured by kit. Results： Compared with the control group, increased liver coefficient, serum level of AST, ALT and GGT, MDA content in liver tissues, and decreased body weight and SOD activity of mice in chromium exposed group were measured （P 〈 0.05 ）. After MT protection, the body weight of mice were recovery, with decreased liver organ coefficient, decreased serum level of AST, ALT and GGT, increased SOD activity and reduced MDA content. There was doseeffect relationship betweent the degree of recovery and MT （P 〈 0.05 ）. Conclusion： Chromium （Cr6＋） has damaged on mice liver, and there is protective effect of MT, of which mechanism is related to antioxidant effect.