白细胞介素-17与细胞核因子-κB受体活化因子配体在强直性脊柱炎患者血清中表达的研究

The study on the expression of interleukin-17 and receptor activator of nuclear factors κB-ligand in serum of patients with ankylosing spondylitis

目的 探讨白细胞介素（IL）-17、细胞核因子-κB受体活化因子配体（RANKL）及骨保护素在强直性脊柱炎（AS）发病机制中的作用及意义.方法 采用酶联免疫吸附试验（ELISA）检测44例AS患者及15名同期健康体检者血清IL-17、RANKL及骨保护素,流式细胞术检测人类白细胞抗原（HLA）-B27；统计学方法采用t检验、Spearman相关分析.结果 ①AS患者血清IL-17 [（45±12） pg/ml]、RANKL[（354±96） pg/ml]表达水平及RANKL/骨保护素[（6.5±3.4） pg/ml]比值均高于对照组（t=11.18,P＜0.05;t=18.66,P＜0.05；t=15.48,P＜0.05）,并且中晚期血清RANKL表达水平高于早期血清RANKL水平（t=47.02,P＜0.05）；中晚期血清骨保护素表达水平显著高于早期,差异有统计学意义（t=15.48,P＜0.05）.②AS组血清IL-17水平与RANKL呈正相关（r=0.532,P=0.021）,血清IL-17、RANKL与Bath AS病情活动指数（BASDAI）均呈正相关（r=0.625,P=0.023；r=0.431,P=0.016）.结论 IL-17与RANKL可能在AS发病中发挥作用.

Objective To investigate the role and value of interleukin （IL）-17,receptor activator of nuclear factors κB-ligand （RANKL）,osteoprotegerin （OPG） in the pathogenesis of AS by detecting the expression of IL-17,RANKL,OPG.Methods IL-17,RANKL,OPG in 44 AS and 15 healthy controls were measured by ELISA.HLA-B27 was measured by flow cytometry.T-test,Spearman＇s correlation analysis were used for statisical analysis.Results ① Serum IL-17 [（45±12） pg/ml],RANKL [（354±96） pg/ml] levels and RANKL/OPG [（6.5±3.4） pg/ml] ratio were significantly higher in AS patients than those in normal controls （t=11.18,P＜0.05; t=18.66,P＜0.05; t=15.48,P＜0.05）,and the serum RANKL levels was higher in AS patients in middle and late period than that in AS patients in early stage （t=47.02,P＜0.05）.The serum OPG levels was higher in AS patients in middle and late period than that in AS patients in early stage （t=15.48,P＜0.05）.② There were positive linea correletion,respectively,between the serum levels of IL-17 and RANKL （r=0.532,P=O.021）,and between serum levels of IL-17 and disease activities of BASDAI （r=0.625,P=0.023）,between serum levels of RAN KL and disease activities of BASDAI （r=0.431,P=0.016）.Conclusion The surem levels of IL-17 and RANKL are increased in AS patient.