IL-1β对BeWo细胞ABCG2 mRNA表达的影响

Effect of IL-1β on ABCG2 mRNA Expression in BeWo Cells

目的:探讨人滋养细胞ABC膜转运蛋白家族G2蛋白(ABCG2)mRNA的表达与白细胞介素-1β(IL-1β)的关系。方法:体外培养人胎盘滋养细胞BeWo,分为IL-1β干预组和空白对照组,每组重复3次,干预组分别于12、24、48、72小时收集BeWo细胞,抽提总RNA。采用实时荧光定量聚合酶链反应(PCR)检测两组细胞ABCG2 mRNA表达。结果:实时荧火定量PCR检测干预12、24、48、72小时ABCG2 mRNA表达量:IL-1β干预组1.19±0.02、1.90±0.04、2.59±0.03、2.98±0.07(×106copies/μg RNA),空白对照组1.00±0.03、1.01±0.03、0.99±0.03、1.04±0.02(×106copies/μg RNA),IL-1β干预组ABCG2 mRNA表达量显著高于相应时间点空白对照组的表达(F=6657.235,P=0.000)。IL-1β干预组后一时间点的表达量显著高于前一时间点的表达量(F=783.772,P=0.000),且IL-1β干预各组的表达量显著高于任一时间点空白对照组的表达量(F=744.127,P=0.000)。结论:IL-1β能增强BeWo细胞ABCG2的表达,提示IL-1β可能在转录水平上调ABCG2 mRNA表达。

Objective：To determine the relation between IL-1β and the expression of ABCG2 mRNA in BeWo cells. Methods： Invitro cultured BeWo cells were treated with or without IL-1β for various periods（ 12 h,24 h,48 h,72h）. The ABCG2 mRNA were quantified by real-time PCR. Results ：The ABCG2 mRNA were 1.19 ±0. 02,1.90 ±0.04, 2.59 ＋0.03,2.98 ±0.07 in IL-1β group,and 1.00 ±0. 03,1.01 ±0.03±0. 99 ±0. 03,1.04 ±0.02 in control groups（ x 106copies/tJg RNA） o Relative quantitative PCR showed a statistically significant（ F= 6657. 235, P =0. 000）increase in ABCG2 m RNA transcription in BeWo cells treated with I L-1 at 12 h,24 h,48 h and 72 h later. And the transcription level was increased remarkably with correspondence to time（ F=783. 772, P=0. 000）o Expression of ABCG2 mRNA in each group of IL-1β groups is higher than that of the control groups（ F = 744. 127, P = 0. 000 ） o Conclusions： ABCG2 mRNA transcription significantly increased in BeWo cells treated with IL-115o IL-1β may activate the transcription of ABCG2 mRNA.