PBRM1和P53在子宫内膜癌的表达及临床意义研究

Expressions and Clinical Significances of PBRM1 and P53 in Endometrial Carcinoma

目的:探讨PBRM1和抑癌基因(P53)在子宫内膜癌中的表达及其临床意义。方法:应用RT-PCR、Western blot、免疫组化方法对105例子宫内膜癌组织及癌旁正常组织(对照组)进行PBRM1和P53mRNA和蛋白表达水平的检测,结果:子宫内膜癌组织PBRM1mRNA及P53 mRNA表达阳性率分别为71.43%、81.90%,对照组分别为28.57%、18.10%,子宫内膜癌组织表达高于对照组(P<0.01)。子宫内膜癌组织PBRM1和P53蛋白表达阳性率分别为62.86%和46.67%,对照组分别为45.71%和7.62%,子宫内膜癌组织表达高于对照组(P<0.05)。PBRM1和P53蛋白阳性表达与组织学类型、浸润深度、淋巴结转移、病理分级、临床TNM分期有关(P<0.05)。PBRM1和P53的表达呈正相关(r=0.205,P<0.05)。结论:PBRM1和P53在子宫内膜癌的表达增高,其表达水平随着子宫内膜癌组织的病理分级和临床分期的增高而增高,可能与肿瘤的生物学行为有一定关系,且两者可能有协同作用。

Objective：To evaluate the expressions and clinical significances of PBRM1 and P53 in endom- etrial carcinoma. Methods：RT-PCR,Western blot,and immunohistochemistry were used to determine the ex- pressions of PBRM1 and P53 rnRNA and protein in 105 cases of endometrial carcinoma. Results：In endom- etrial carcinoma group,the positive rate of PBRM1 and P53 mRNA was 71.43% and 81.90% ,respectively; and was 28, 57% and 18. 10% in control group（ P〈0. 01 ）. In endometrial carcinoma group,the positive rate of PBRM1 and P53 protein was 62.86% and 46.67% ,respectively;and was 45.71% and 7. 62% in control group（ P 〈 0.05）. PBRM1 and P53 expressions were positively correlated with the histological type, depth of invasion, lymph node metastasis, histological grade, and clinical TNM stage（ P 〈 0.05）. Expression of PBRM1 was positively correlated with expression of P53 （r =0. 205, P 〈 0.05）. Conclusions：Expressions of PBRM1 and P53 increase in endometrial carcinoma,which were correlated with with pathological grade and clinical stage,suggesting some activities in the biological behavior of the tumor.