人β-防御素3的原核表达及生物活性研究

Prokaryotic expression of human beta-defensin 3 and the biological activity assay

为研究人β-防御素3(hBD3)蛋白的生物活性,本研究将构建的pET-hBD3重组质粒转染大肠杆菌BL21(DE3)感受态细胞,通过IPTG诱导表达,检测结果显示在36℃、0.7 mmol/L/IPTG诱导6 h表达量达到峰值。SDS-PAGE检测显示表达的重组蛋白约26 ku,通过抑菌试验检测,hBD3蛋白对革兰氏阴性大肠杆菌、革兰氏阳性金黄色葡萄球菌均有抑菌作用,其中对革兰氏阳性金黄色葡萄球菌的抑菌作用更明显。本实验为进一步研究hBD3的作用奠定基础。

To study human beta-defensin 3 （hBD3）, the recombinant expression plasmid of pET-hBD3 was constructed for expression in Escherichia coli. The recombinant hBD3 （rhBD3） was efficiently expressed under the optimized conditions of 36 ~C for 6 hours inducing with 0.7 mmol/L of IPTG. SDS-PAGE assay indicated that the molecular weight of expressed rhBD3 was about 26 ku. The antimicrobial bioactivity tests showed that the rhBD3 had antirnicrobial bioactivity against S.aureus and E.coli, and antimicrobial bioactivity against S.aureus was more efficient.