健脾清化方调节局灶节段硬化大鼠炎症信号通路的机制

Effect of Jianpi Qinghua decoctions on interleukin-6 and monocyte chemotactic protein-1 in rats with focal segmental glomurular sclerosis nephropathy: role of the inflammatory signaling pathways

目的从阻断炎症细胞生物功能角度,探讨健脾清化方对局灶节段硬化(FSGS)模型大鼠肾纤维化的影响。方法左侧肾切除加尾静脉注射阿霉素致大鼠肾脏FSGS,观察健脾清化方对模型大鼠白细胞介素6(IL-6)、单核细胞趋化蛋白-1(MCP-1)、细胞间黏附分子(ICAM-1)表达的影响。结果大鼠肾小管间质IL6表达,健脾清化方组、健脾方组、清化方组、尿毒清组明显低于模型组(P<0.01)。大鼠肾小管间质MCP-1表达,健脾清化方组、健脾方组、清化方组、尿毒清组较模型组明显下降(P<0.01);健脾清化方组较尿毒清组下降(P<0.05)。大鼠肾小管间质ICAM-1表达,与模型组相比,健脾清化方组、清化方组、尿毒清组明显下降(P<0.01),健脾方组较模型组下降(P<0.05);健脾清化方组较尿毒清组下降(P<0.05)。大鼠肾小管间质MCP-1、ICAM-1荧光强度,与模型组相比,健脾清化方组、健脾方组、清化方组、尿毒清组明显减弱(P<0.01),与尿毒清组比较,健脾清化方组、清化方组荧光强度明显减弱(P<0.01)。健脾清化方能显著降低IL6、MCP-1、ICAM-1表达,且对MCP-1、ICAM-1的效果优于尿毒清组。结论健脾清化方对FSGS模型大鼠肾脏的炎症损伤和肾纤维化有一定的改善作用,该机制可能与健脾清化方终止IL-6的信号通路有关。

Objective To study the effect of Jianpi Qinghua decoctions in ameliorating kidney fibrosis in rats with focal segmental glomurular sclerosis （FSGS） nephropathy in light of blocking the functions of the inflammatory cells. Methods Rat models of FSGS nephropathy were established by left nephrectomy and intravenous injection of adriamycin and treated with Jianpi Qinghua decoction. The renal expressions of interleukin-6 （IL-6）, monocyte chemotactic protein 1 （MCP-1）, and intercellular adhesion molecular-1 （ICAM-1） were detected. Results Treatment with fianpi Qinghua decoction, Jianpi decoction, Qinghua decoction, and Niaoduqing particles all significantly lowered renal tubulointerstitial expressions of IL-6 in the model rats （P〈 0.01）. Renal tubulointerstifial MCP-1 expressions were all lowered significantly in the rats by treatments with Jianpi Qinghua decoction, Jianpi decoction, QingHua decoctions, and Niaoduqing particles （P〈0.01）, and Jianpi Qinghua decoction produced a stronger effect than Niaoduqing particles （P〈0.05）. Jianpi Qinghua decoction, Jianpi decoction, Qinghua decoctionsand and Niaoduqing particles all significantly decreased renal tubulointersfitial MCP-1 and ICAM-1 expressions （P〈0.01）, and Jianpi Qinghua and Qinghua decoctions both showed stronger effects than Niaoduqing particles （P〈0.01）. Jianpi Qinghua decoctionssignificantly suppressed the expressions of IL-6, MCP-1, and ICAM-1 and produced stronger effects than Niaoduqing particles on MCP-1 and ICAM-1 expressions. Conclusion Jianpi Qinghua decoctions can ameliorate inflammatory injury and lower the levels of inflammatory factors in rats with FSGS nephropathy, the mechanism of which is associated with inhibiting IL-6 signaling pathway.