胃癌细胞中JAK-STAT和ras-MAPK信号通路相关基因的表达及意义

Gene Expression and Significance of JAK-STAT and ras-MAPK Signaling Pathways in Gastric Cancer Cells

目的通过检测不同分化程度胃癌细胞中ras、STAT3、p38MAPK的表达,分析各基因表达的关系。方法采用RT-PCR和蛋白印迹分析法检测人胃癌细胞株MKN45、SGC7901、MKN28中ras、STAT3、p38MAPK核酸和蛋白的表达水平。结果人低分化胃癌细胞株MKN45中ras、STAT3、p38MAPK的mRNA表达水平明显高于人中分化胃癌细胞株SGC7901(P<0.05)和人高分化胃癌细胞株MKN28(P<0.01);人低分化胃癌细胞株MKN45中ras、STAT3、p38MAPK的蛋白表达水平明显高于人中分化胃癌细胞株SGC7901(P<0.05)和人高分化胃癌细胞株MKN28(P<0.01)。ras与STAT3(r=0.94,P<0.01)、p38MAPK(r=0.89,P<0.01)mRNA的表达均呈显著正相关;STAT3和p38MAPK mRNA的表达呈正相关(r=0.76,P<0.05);ras与STAT3蛋白的表达水平呈显著正相关(r=0.97,P<0.01);ras与p38MAPK蛋白的表达水平呈正相关(r=0.72,P<0.05);STAT3和p38MAPK蛋白的表达亦呈正相关(r=0.69,P<0.05)。结论 ras、p38MAPK和STAT3的过度表达在胃癌的发生、发展、分化过程中起重要作用,ras-MAPK和JAK-STAT通路的平衡失调可能是导致胃癌发生的重要原因。

Objective To investigate the expression and correlation ofras, signal transducers and activators of transcription 3 （STAT3） and p38MAPK in gastric cancer cells. Methods RT-PCR and Western blot methods were used to detect the expression of ras, STAT3 and p38MAPK of gastric cancer cell lines MKN45,SGC7901 and MKN28. Results The mRNA expression levels of ras, STAT3, p38MAPK in human poorly differentiated gastric cancer cell line MKN45 were significantly higher than those in both human moderately differentiated gastric cancer cell line SGC7901 （P 〈0.05） and human well-differentiated gastric cancer cell line MKN28 （P〈0.01）. The protein levels of ras, STAT3, p38MAPK in human poorly differentiated gastric cancer cell line MKN45 were significantly higher than those in human moderately differentiated gastric cancer cell line SGC7901 （P 〈0.05） and human well-differentiated gastric cancer cell line MKN28 （P 〈0.01）. There was a significant positive correlation between mRNA expression of ras, STAT3 （r=0.94, P 〈0.01 ） and p38MAPK （r=0.89,P〈0.01） . The mRNA expression of STAT3 and p38MAPK was positively correlated with each other （r=0.76,P〈0.05）. There was a significant positive correlation between protein expression of ras and STAT3 （r=0.97,P〈0.01） . The protein levels of ras and p38MAPK was positively correlated （r=0.72, P〈0.05） .The protein levels of STAT3 and p38MAPK was positively correlated（ r=0.69, P〈0.05）.Conclusion The overexpressions of ras, STAT3 and p38MAPK play an important role in carcinogenesis, development and differentiation of gastric cancer, ras-MAPK and JAK-STAT pathway imbalance may be an important cause of gastric cancer.